Visible Continuum Source for Fluorescence Excitation

用于荧光激发的可见连续光源

• 批准号: 6833603
• 负责人: THOMAS J KANE
• 金额: $ 8.14万
• 依托单位: LIGHTWAVE ELECTRONICS CORPORATION
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-24 至 2005-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6833603
• 关键词: bioengineering /biomedical engineering; bioimaging /biomedical imaging; biomedical equipment development; fluorescence; imaging /visualization /scanning; lasers; light emission; optics; visible light

DESCRIPTION (provided by applicant): We propose to develop a light source having a spectrum that spans the visible wavelengths, with a beam quality similar to a laser. Our goal is to cover the wavelengths from 400 nm- 700 nm, with at least 1 mW in any 1 nm slice of the spectrum. The light source will be based on an inexpensive diode-pumped mode-locked laser, the output of which is spectrally broadened into a continuum by a highly nonlinear, single-mode, fused-silica fiber. The output of the fiber will be pulses of diffraction-limited white light, with a pulse repetition rate near 100 MHz. Such a high repetition rate is equivalent to continuous light for virtually all instrument applications. Such a source would have many laboratory uses, for example in fluorescence imaging. The spectrum of the proposed source could be filtered to obtain a laser-like beam at any desired visible wavelength. This flexibility would make fluorescence confocal microscopes much more powerful tools, since they could be used at any excitation wavelength.

描述（由申请人提供）：我们提出开发具有跨越可见波长的光谱的光源，其光束质量类似于激光。我们的目标是覆盖400 nm- 700 nm的波长，在光谱的任何1 nm切片中至少有1 mW。光源将基于廉价的二极管泵浦锁模激光器，其输出通过高度非线性的单模熔融石英光纤在光谱上展宽成连续谱。光纤的输出将是衍射极限白色光的脉冲，脉冲重复率接近100 MHz。如此高的重复率对于几乎所有的仪器应用来说都相当于连续光。这样的源将具有许多实验室用途，例如在荧光成像中。所提出的源的光谱可以被过滤以获得在任何期望的可见波长处的类激光光束。这种灵活性将使荧光共聚焦显微镜更加强大的工具，因为它们可以在任何激发波长下使用。