RNA expression profiles for Xenopus

描述（由申请人提供）：这个小企业创新研究的第一阶段申请描述了如何为使用Xenopus的研究人员提供寡核苷酸微阵列幻灯片和服务，Xenopus是一个对生物学做出巨大贡献的模型系统。许多研究小组很少或根本没有办法获得基因组学研究的图书馆，其中最突出的是研究两栖动物的研究人员。为了满足这一需求，我们提出建立非洲爪蟾和热带爪蟾基因的寡核苷酸文库。研究将进行比较全球基因组表达谱的X. laevis和X.热带。拟议的工作将使爪蟾研究人员能够将历史上重要的X. laevis系统的数据与相对较新的X. tropical系统相结合，后者为爪蟾系统提供了进行遗传学和相关研究的独特特征。将通过比较微阵列研究中选定基因的基因表达水平与先前工作和/或传统方法确定的水平来进行拟议工作的有效性。本研究为第二阶段提供了基础，第二阶段将扩展和开发微阵列产品和服务，用于X. laevis和X. tropical alis以及其他重要和被忽视的模型系统。拟议中的工作将促进基因组学，并开发利润丰厚的商业和学术市场。

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I application describes how oligonucleotide microarray slides and services will be provided for researchers using Xenopus, a model system that has contributed tremendously to biology. Many research groups have little or no means for obtaining libraries for genomics studies and prominent among them are researchers who work with amphibians. To fulfill this need, we propose to generate oligonucleotide libraries representing genes from Xenopus laevis and X. tropicalis. Studies will be carried out to compare the global genomic expression profiles of X. laevis and X. tropicalis. The proposed work will allow Xenopus researchers to integrate the data from the historically significant X. laevis system with the relatively new X. tropicalis system, which offers the singular feature for the Xenopus system to carry out genetics and related studies. The efficacy of the proposed work will be carried out by comparing gene expression levels of selected genes from the microarray studies to levels determined from previous work and/or by conventional methods. The proposed research provides the basis for Phase II, which is to extend and develop the microarray products and services for X. laevis and X. tropicalis as well as other important and overlooked model systems. The proposed work will promote genomics and exploit lucrative commercial and academic markets.