RNA expression profiles for Xenopus

非洲爪蟾的 RNA 表达谱

• 批准号: 6787545
• 负责人: MICHAEL L HOWELL
• 金额: $ 10万
• 依托单位: PROTEIN EXPRESS, INC.
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-01-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6787545
• 关键词: RNA; Xenopus; biotechnology; developmental genetics; gene expression; gene expression profiling; genetic library; genetic models; genome; microarray technology; model design /development; molecular biology information system; nucleic acid sequence; oligonucleotides; species difference

DESCRIPTION (provided by applicant): This Small Business Innovation Research Phase I application describes how oligonucleotide microarray slides and services will be provided for researchers using Xenopus, a model system that has contributed tremendously to biology. Many research groups have little or no means for obtaining libraries for genomics studies and prominent among them are researchers who work with amphibians. To fulfill this need, we propose to generate oligonucleotide libraries representing genes from Xenopus laevis and X. tropicalis. Studies will be carried out to compare the global genomic expression profiles of X. laevis and X. tropicalis. The proposed work will allow Xenopus researchers to integrate the data from the historically significant X. laevis system with the relatively new X. tropicalis system, which offers the singular feature for the Xenopus system to carry out genetics and related studies. The efficacy of the proposed work will be carried out by comparing gene expression levels of selected genes from the microarray studies to levels determined from previous work and/or by conventional methods. The proposed research provides the basis for Phase II, which is to extend and develop the microarray products and services for X. laevis and X. tropicalis as well as other important and overlooked model systems. The proposed work will promote genomics and exploit lucrative commercial and academic markets.

描述（由申请人提供）：此小型企业创新研究第一阶段申请描述了如何使用Xenopus（一种对生物学做出巨大贡献的模型系统）为研究人员提供寡核苷酸微阵列载玻片和服务。许多研究小组很少或根本没有办法获得基因组学研究的文库，其中最突出的是研究两栖动物的研究人员。为了满足这一需要，我们建议产生代表非洲爪蟾和非洲爪蟾基因的寡核苷酸文库。热带植物将进行研究以比较X的全球基因组表达谱。laevis和X.热带植物这项拟议中的工作将允许爪蟾研究人员整合来自历史上重要的X。laevis系统与相对较新的X. tropicalis系统，为爪蟾系统进行遗传学及相关研究提供了独特的功能。将通过比较来自微阵列研究的选定基因的基因表达水平与从先前工作和/或通过常规方法确定的水平来进行所提出的工作的功效。这项研究为第二阶段的工作奠定了基础，第二阶段的工作是扩展和开发X的微阵列产品和服务。laevis和X. Tropicalis以及其他重要和被忽视的模型系统。拟议的工作将促进基因组学并开发利润丰厚的商业和学术市场。