Structural Organization of the Superior Colliculus

上丘的结构组织

• 批准号: 6835670
• 负责人: WILLIAM Charles HALL
• 金额: $ 34.65万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-08-01 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6835670
• 关键词: Structural; Organization; Superior; Colliculus

EXCEED THE SPACE PROVIDED. One of the great challenges in neurobiology is to understand how sensory and motor systems are integrated to produce behavior. The juxtaposition of cells with sensory and motor properties has made the superior colliculus a fruitful model for studying this process. By applying methods new to the study of the superior colliculus, including in vitro whole-cell patch-clamp recording and photostimulation, we are analyzing for the first time at the cellular level the intrinsic circuitry that links its sensory and motor functions. By filling the cells during recording with neuronal tracers and by pre-labeling them with retrogradely transported tracers, their physiology is correlated with their morphology and connections. =Using this approach, we demonstrated that the superficial collicular layer, which encodes the location of visual stimuli, sends excitatory signals to a deeper layer of premotor cells, which generates commands for visually guided shifts in the direction of gaze. The premotor layer also receives a dense cholinergic input that arises from the same brainstem cells that modulate the flow of sensory information through the visual part of the thalamus to the cortex. Our first Specific Aim is to test the idea that this cholinergic input coordinates the enhancement of sensory signals transmitted to the cortex with the initiation of orienting movements toward visual stimuli. Our second Specific Aim is to test the idea that modulation of premotor activity by long-range inhibition - between the right and left colliculi and between rostral fixation and caudal gaze shifting neurons - permits the activation of premotor cells that command head and eye movements toward visual stimuli in one direction while attenuating the activity of premotor cells that would command competing movements toward stimuli in other directions. These experiments will contribute to our understanding of sensorimotor functions in the normal and diseased brain. PERFORMANCE SITE ========================================Section End===========================================

超出所提供的空间。神经生物学的巨大挑战之一是了解感觉和运动系统如何整合以产生行为。具有感觉和运动特性的细胞的并置使上丘成为研究这一过程的富有成效的模型。通过应用上丘研究的新方法，包括体外全细胞膜片钳记录和光刺激，我们首次在细胞水平上分析连接其感觉和运动功能的内在电路。通过在记录过程中用神经元示踪剂填充细胞并用逆行运输的示踪剂预先标记它们，它们的生理学与其形态和连接相关联。 =使用这种方法，我们证明了编码视觉刺激位置的表层丘层将兴奋信号发送到更深层的前运动细胞，后者生成视觉引导的凝视方向移动的命令。前运动层还接收来自相同脑干细胞的密集胆碱能输入，这些脑干细胞调节通过丘脑视觉部分到皮质的感觉信息流。我们的第一个具体目标是测试这样的想法：胆碱能输入协调传输到皮层的感觉信号的增强，并启动针对视觉刺激的定向运动。我们的第二个具体目标是测试这样一种想法，即通过远程抑制（左右丘之间以及头侧固定和尾侧凝视转移神经元之间）调节前运动活动，允许激活前运动细胞，这些前运动细胞指挥头部和眼睛朝一个方向的视觉刺激运动，同时减弱前运动细胞的活动，这些前运动细胞指挥朝其他方向的刺激进行竞争性运动。这些实验将有助于我们了解正常和患病大脑的感觉运动功能。表演网站==========================================章节结束===============================================