Understanding the mechanism of actin-microtubule co-regulation underlying neurodegeneration in motor neurons

了解肌动蛋白-微管共同调节运动神经元神经变性的机制

• 批准号: 2441790
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2441790
• 关键词: Understanding; mechanism; actin; microtubule; co

Disrupted microtubule dynamics are a key mechanism underlying the selective vulnerability of neurons across many different genetic motor neuron diseases. Microtubules facilitate the long range transport of cargo along axons and stabilise the endoplasmic reticulum and mitochondria distribution along neurons. This project will investigate the microtubule based molecular causes of Hereditary Spastic Paraplegia type 8 (HSP8), a motor neuron disease originating from point mutations in the protein Strumpellin. The student will address this question using a wide range of techniques including super-resolution live cell microscopy of neurons; cell biological analysis of the signalling pathways and in vitro reconstitution of microtubule dynamics.

微管动力学的破坏是神经元在许多不同遗传性运动神经元疾病中选择性脆弱性的关键机制。微管促进货物沿着轴突的长距离运输，并稳定内质网和线粒体沿沿着神经元的分布。该项目将研究遗传性痉挛性截瘫8型（HSP 8）的微管分子原因，HSP 8是一种运动神经元疾病，起源于蛋白质Strumpelin的点突变。学生将使用广泛的技术来解决这个问题，包括神经元的超分辨率活细胞显微镜;信号通路的细胞生物学分析和微管动力学的体外重建。