Regulated proteolysis in Caulobacter cell cycle

18. 奖学金培训和职业目标姓名（最后，第一，中间首字母缩写）陈，约瑟夫C.机构导师哈维马德学院南希V.哈姆利特哈佛大学乔纳森贝克威斯机构/公司主管雇主阿斯利康爱德布尔曼提议的奖学金将加深我作为一名研究科学家的训练。这也将增强我对微生物如何调节细胞和发育事件的理解。我将学习结合不同学科的工具和概念——如遗传学、生物化学、生物信息学和细胞生物学——来研究细菌细胞中调节信号的整合。在完成奖学金后，我计划继续研究控制细胞分化和细胞周期进程的分子机制。这次博士后培训将为我成为一名独立研究者做准备。19. 姓名及学位：露西尔·夏皮罗博士职位/等级：发育生物学教授主要研究方向：细胞发育，细胞周期调控，蛋白质和染色体定位it_1: _1__1，_m: u:（.m.）l-v = _ 22。描述（请勿超出篇幅）：月牙根杆菌协调细胞周期进程和细胞分化，遵循精确的发育程序。为了确保这个程序的忠实执行，细菌采用了多种调节策略，其中一个关键是在特定的时间和地点对特定蛋白质进行蛋白质水解。本研究旨在确定膜结合金属蛋白酶在发育事件调控中的作用。月牙菇中的许多这些事件涉及动态定位的跨膜蛋白，而整体膜蛋白酶可能影响这些蛋白的定位或稳定性。评估是否和如何选择金属蛋白酶参与细胞周期调节。并对其蛋白水解活性和靶点进行分析。了解蛋白质水解如何控制这种模式生物的关键细胞过程将有助于阐明在人类正常细胞增殖过程中类似机制是如何运作的。小灵通416 - 1(启12/98)表单页面2 BB cc个人NRSA应用程序目录 ======================================== 节结束 ===========================================

18. GOALS FOR FELLOWSHIP TRAINING AND CAREER NAME (Last, first, middle initial) Chen, Joseph C. INSTITUTION MENTOR Harvey Mudd College Nancy V. Hamlett Harvard University Jonathan Beckwith INSTITUTION/COMPANY SUPERVISO_EMPLOYER AstraZeneca Ed Buurman The proposed fellowship will deepen my training as a research scientist. It will also enhance my understanding of how cellular and developmental events are regulated in microorganisms. I will learn to combine tools and concepts from different disciplines--such as genetics, biochemistry, bioinformatics, and cell biology--to study the integration of regulatory signals in a bacterial cell. After completing the fellowship, I plan to continue research on the molecular mechanisms that control cellular differentiation and cell cycle progression. This postdoctoral training will prepare me for a career as an independent investigator. 19. NAME AND DEGREE(S) Lucille Shapiro, Ph.D. 20. POSITION/RANK Professor of Developmental Biology 21. RESEARCH INTERESTS/AREAS development, cell cycle control and regulation, localization of proteins and chromosomes it_1:_1i1_,_m: u:(.m.l-v=_ 22. DESCRIPTION (Do not exceed space provided) The bacterium Caulobacter crescentus coordinates cell cycle progression and cellular differentiation to follow a precise developmental program. To ensure faithful execution of this program, the bacterium employs multiple regulatory strategies, a key one being proteolysis of specific proteins at defined times and locations. This proposal aims to determine the roles of membrane-bound metalloproteases in the regulation of developmental events. Many of these events in C. crescentus involve dynamically positioned transmembrane proteins, and integral membrane proteases may influence the localization or stability of such proteins. An assessment will be made of whether and how select metalloproteases participate in cell cycle regulation. Their proteolytic activities and targets will also be analyzed. Understanding how proteolysis controls critical cellular processes in this model organism will help elucidate how similar mechanisms operate during normal cell proliferation in humans. PHS 416-1 (Rev. 12/98) Form Page 2 BB cc Individual NRSA Application Table of Contents ========================================Section End===========================================