Micromechanical Integrated DNA Analysis Technology

项目描述：（由申请人提供）该计划项目的重点是开发可集成的微加工DNA分析组件。 这一延续提案将开发用于基因分型和测序的纳升级DNA样本处理和分析技术。 遗传分析的微流体、分离和反应组件制造在硅、玻璃和/或聚合物衬底上。 为了优化廉价的设计、测试和生产，这些器件采用了可拆卸的结构技术。 该提案有三个方案项目组成部分，目标如下： 项目1。 组件开发和改进。 识别单个组分，并评价其在集成系统中的适用性。 在有限数量的组件设计上进行密集的工程改进。 项目2. 先进的元件和器件研究。 先进的光学探测器，微流控通道，新的制造方法，流体控制，表面相互作用和电泳所需的基本工程信息。 生物样品处理和DNA生物化学程序是为微尺度集成系统开发的。 核心单位。 制造和仪器支持。 维护一个中央装配和测试设施，可以快速构建和测试每个项目的原型和组件。 经验丰富的研究工程师为整个计划提供制造技能，知识和培训。

DESCRIPTION: (provided by applicant) The Program Project is focused on the development of integratable microfabricated DNA analysis components. This continuation proposal will develop nanoliter-scale DNA sample handling and analysis technologies for genotyping and for sequencing. The microfluidic, separation, and reaction components of genetic analysis are fabricated on silicon, glass, and/or polymer substrates. To optimize inexpensive design, testing, and production, the devices are made using photolithographic construction techniques. The proposal has three Program Project components, with the following goals: Project 1. Component Development and Refinement. Individual components are identified and are evaluated for suitability in an integrated system. Intensive engineering refinement occurs on a restricted number of component designs. Project 2. Advanced Component and Device Research. The basic engineering information required for advance optical detectors, microfluidic channels, novel fabrication methods, fluid control, surface interactions, and electrophoresis are obtained. Biological sample processing and DNA biochemistry procedures are developed for microscale integrated systems. Core Unit. Fabrication and Instrumentation Support. A central assembly and testing facility is maintained that can rapidly build and test prototypes and components for each of the projects. Experienced research engineers provide fabrication skills, knowledge, and training for the entire Program.