Micromechanical Integrated DNA Analysis Technology

描述：（由申请人提供）该项目专注于可集成微制造DNA分析组件的开发。这一继续提案将开发纳米级DNA样品处理和分析技术，用于基因分型和测序。遗传分析的微流控、分离和反应组件是在硅、玻璃和/或聚合物衬底上制造的。为了优化廉价的设计、测试和生产，这些设备采用光刻构造技术制造。该提案有三个项目组成部分，目标如下：

DESCRIPTION: (provided by applicant) The Program Project is focused on the development of integratable microfabricated DNA analysis components. This continuation proposal will develop nanoliter-scale DNA sample handling and analysis technologies for genotyping and for sequencing. The microfluidic, separation, and reaction components of genetic analysis are fabricated on silicon, glass, and/or polymer substrates. To optimize inexpensive design, testing, and production, the devices are made using photolithographic construction techniques. The proposal has three Program Project components, with the following goals: Project 1. Component Development and Refinement. Individual components are identified and are evaluated for suitability in an integrated system. Intensive engineering refinement occurs on a restricted number of component designs. Project 2. Advanced Component and Device Research. The basic engineering information required for advance optical detectors, microfluidic channels, novel fabrication methods, fluid control, surface interactions, and electrophoresis are obtained. Biological sample processing and DNA biochemistry procedures are developed for microscale integrated systems. Core Unit. Fabrication and Instrumentation Support. A central assembly and testing facility is maintained that can rapidly build and test prototypes and components for each of the projects. Experienced research engineers provide fabrication skills, knowledge, and training for the entire Program.