REGULATION OF THE HUMAN CD34 STEM CELL GENE

超出提供的空间。人CD34抗原在人的造血干细胞上特异表达，而在成熟的血细胞上不表达，CD34细胞可以重建所有细胞系的正常造血。CD34仍然是定义最好的人类干细胞标记物，因此是干细胞基因表达的模型。以前的研究已经定义了人和小鼠CD34的一些启动子元件，但对这个和其他干细胞基因的调控仍然知之甚少，最近分离出了人CD34在体内表达所需的末端元件。本项目的目标是继续对CD34的调控元件进行定义和分析，并对CD34基因中那些可以指导异源基因的干细胞表达的元件进行鉴定。这些元件的特性可能导致开发新的基因治疗载体，以及对干细胞中调控基因的因素有新的理解。因此，本研究的具体目的是从以下几个方面对CD34基因的调控元件进行鉴定和研究：1.进一步鉴定调控人CD34基因在干细胞中表达的重要调控元件。2.鉴定调控这些元件的DNA结合蛋白。1使用表达Cre重组酶的CD34调控元件和四环素反式激活剂，使异源基因在干细胞室的表达具有组织特异性和可调性。表演网站========================================Section End===========================================

EXCEED THE SPACE PROVIDED. The human CD34 antigen is expressed specifically on human hematopoietic stem cells and not on mature blood cells, and CD34+ cells can reconstitute normal hematopoiesis of all cell lineages. CD34 is still the best defined human stem cell marker and is therefore a model for stem cell gene expression. Previous studies have defined some of the promoter elements for human and murine CD34, but the regulation of this and other stem cell genes is still very poorly understood, The distal elements necessary for human CD34 expression in vivo were recently isolated. The goal of this project is to continue to define and analyze the control elements of CD34, and to characterize those elements of the CD34 gene which can direct stem ceil expression of heterologous genes. The characterization of these elements could lead to the development of new vectors for gene therapy, as well as new understanding of the factors regulating genes in stem cells. Therefore, the specific aims are to characterize and study the regulatory elements of CD34 in the following steps: 1. To further characterize the important control elements regulating human CD34 gene expression in stem cells. 2. To characterize DNA binding proteins which regulate these elements. 1 To use CD34 regulatory elements expressing Cre recombinase and the tetracycline transactivator to enable tissue specific, regulatable expression of heterologous genes in the stem cell compartment. PERFORMANCE SITE ========================================Section End===========================================