Next-generation biosensors for real-time, enhanced sensitivity antigen detection

用于实时、增强灵敏度抗原检测的下一代生物传感器

• 批准号: 2449938
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2449938
• 关键词: Next; generation; biosensors; real; time

The ability to measure biomarkers and trace analytes both specifically and selectively at the single-molecule level in biological fluids has the potential to transform the diagnosis, real-time screening, analyte seining for specific hazardous materials of high-profile defence interest. Strategies such as the use of nanopore sensors combined with functional molecular probes have been gaining in prominence not only for sequencing but more recently in screening applications. However, substantial challenges remain in achieving sufficient resolution to distinguish bound from unbound target analytes or in reaching detection of critical analytes that may be present at a very low level in biofluids. The proposed CDT project addresses the above limitations by delivering a new class of biosensors, of importance to the DstL, the research arm of the Ministry of Defence. The platform combines custom molecular probes and dielectrophoretic nanopore sensors, that are capable of selective analyte discrimination in near real-time at sub-pM and fM concentrations.

在生物液体中的单分子水平上特异性和选择性地测量生物标志物和痕量分析物的能力，有可能改变诊断、实时筛查、分析物筛选，以确定具有高度国防利益的特定危险材料。诸如使用纳米孔传感器与功能性分子探针相结合的策略不仅在测序中而且最近在筛选应用中已经变得突出。然而，在实现足够的分辨率以区分结合的与未结合的目标分析物或在达到可能以非常低的水平存在于生物流体中的关键分析物的检测方面仍然存在实质性挑战。拟议的CDT项目通过提供对国防部研究部门DstL具有重要意义的新型生物传感器来解决上述限制。该平台结合了定制分子探针和介电泳纳米孔传感器，能够在亚pM和fM浓度下近实时地选择性分析物区分。