Decoding the role of vascular smooth muscle cell heterogeneity in cardiovascular disease by single cell genomics

通过单细胞基因组学解码血管平滑肌细胞异质性在心血管疾病中的作用

• 批准号: 2453971
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2453971
• 关键词: Decoding; role; vascular; smooth; muscle

Cardiovascular disease is the leading cause of death worldwide. Vascular smooth muscle cells (VSMC) are implicated in the pathogenesis of several types of vascular diseases, such as aneurysm, atherosclerosis, vascular calcification and congenital vascular disease. VSMCs in different blood vessels are heterogeneous, behave differently in healthy and diseased states and derive from different embryonic progenitors. This project will use the latest technologies (single cell RNA-seq, single cell ATAC-seq, spatial transcriptomics) to resolve VSMC subpopulations in the developing human embryo, trace their lineage history and uncover the regulatory programs responsible for their differentiation. The discovery and characterization of disease-relevant transcriptional and chromatin signatures in VSMC subpopulations will help clarifying the basis of human cardiovascular pathology, enable diagnostic solutions for cardiovascular disease and facilitate reverse engineering of human tissues for regenerative purposes.

心血管疾病是全世界死亡的主要原因。血管平滑肌细胞（VSMC）参与了多种血管疾病的发病机制，如动脉瘤、动脉粥样硬化、血管钙化和先天性血管疾病。不同血管中的VSMC是异质的，在健康和疾病状态下表现不同，并且来自不同的胚胎祖细胞。该项目将使用最新技术（单细胞RNA-seq，单细胞ATAC-seq，空间转录组学）来解析发育中的人类胚胎中的VSMC亚群，追踪其谱系历史并揭示负责其分化的调控程序。VSMC亚群中疾病相关转录和染色质特征的发现和表征将有助于阐明人类心血管病理学的基础，实现心血管疾病的诊断解决方案，并促进用于再生目的的人体组织的逆向工程。