Development and application of advanced fluorescence microscopy approaches to study Type VI secretion system effector delivery in vivo.

开发和应用先进的荧光显微镜方法来研究 VI 型分泌系统效应物体内传递。

• 批准号: 2454123
• 金额: --
• 依托单位: University of Dundee
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2020
• 资助国家: 英国
• 起止时间: 2020 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2454123
• 关键词: Development; application; advanced; fluorescence; microscopy

Bacteria exist in polymicrobial communities and the Type VI Secretion System (T6SS) is used by many Gram-negative bacteria to deliver toxic 'effector' proteins into neighbouring bacterial cells as a method of inter-bacterial competition. Understanding the T6SS's mechanism and role in polymicrobial communities, including those important for health and disease, has the potential to increase our ability to develop disease therapies. Using multidisciplinary approaches, we aim to:1. Develop advanced microscopy techniques to visualise individual effectors and the core T6SS components they interact with.2. Identify and characterise the mode of action of a new T6SS effector from a clinical isolate of S. marcescens.3. Build on aims 1 and 2 to gain further mechanistic insight into effector delivery.Our findings will define the activity of a new effector protein, develop new techniques to understand effector mode of action, and address important biological questions about how effector molecules are delivered.

细菌存在于多微生物群落中，并且VI型分泌系统（T6SS）被许多革兰氏阴性细菌用于将毒性“效应器”蛋白递送到邻近的细菌细胞中，作为细菌间竞争的方法。了解T6SS在多微生物群落中的机制和作用，包括对健康和疾病重要的那些，有可能提高我们开发疾病疗法的能力。使用多学科方法，我们的目标是：1。开发先进的显微技术，以可视化个别效应器和核心T6SS组件，他们相互作用。2.鉴定和证实一种新的T6SS效应子的作用模式。marcescens.3.在目标1和2的基础上，进一步深入了解效应子传递的机制。我们的研究结果将定义新效应子蛋白的活性，开发新技术来了解效应子的作用模式，并解决有关效应子分子如何传递的重要生物学问题。