Molecular determinants of nodaviral assembly

描述（由申请人提供）：本提案概述了旨在更好地理解二十面体RNA病毒组装的实验。它侧重于衣壳蛋白和病毒RNA之间的特定相互作用，以及控制病毒基因组识别，凝聚和封装的方面。它解决了多部RNA基因组如何被识别和包装成单个病毒粒子的未解决问题，并试图定义细胞参数在这一过程中的作用。该实验计划利用了一个高度发达的模型系统，该模型系统以含有诺达病毒的二十面体RNA为代表。诺达病毒是一类具有遗传、生化和结构特征的病毒，已被证明在病毒学的基本问题研究中非常容易处理，包括颗粒组装和基因组包装。这个

DESCRIPTION (provided by applicant): This proposal outlines experiments to develop a better understanding of icosahedral RNA virus assembly. It focuses on specific interactions between capsid protein and viral RNA as well as aspects that control recognition, condensation and encapsidation of the viral genome. It addresses the unresolved issue of how multipartite RNA genomes are recognized and packaged into a single virion and it seeks to define the role of cellular parameters in this process. The experimental plan takes advantage of a highly developed model system represented by the icosahedral RNA containing nodaviruses. Nodaviruses are a family of genetically, biochemically and structurally exceptionally well characterized viruses that have proven to be highly tractable for the study of fundamental questions in virology, including particle assembly and packaging of the genome. The specific aims of this project are: (1) to investigate the role of subcellular compartmentalization in the Flock House Virus (FHV) life cycle using biochemical and genetic approaches; (2) to visualize cellular compartmentalization and spatial dynamics during FHV infection by fluorescence microscopy and electron microscopy; (3) to identify mechanistic strategies and molecular elements involved in packaging of the FHV genome and (4) to define the role of FHV coat protein in folding and condensation of viral RNA during assembly.