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CORE--MICROSCOPY AND IMMUNOHISTOCHEMISTRY

CORE--MICROSCOPY AND IMMUNOHISTOCHEMISTRY
核心——显微镜和免疫组织化学
批准号：
7074742
负责人：
JAMES RICHARDSON
金额：
$37.42万
依托单位：
UT SOUTHWESTERN MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
--
资助国家：
美国
项目状态：
未结题
起止时间：
至
来源：
https://reporter.nih.gov/project-details/7074742
关键词：
bioimaging /biomedical imagingbiomedical facilityhistologyimmunocytochemistryin situ hybridizationlaboratory mousemicroscopytissue /cell preparation

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I DESCRIPTION (provided by applicant): The Exercise Core Facility will provide two different approaches, voluntary wheel running and low-frequency tonic pacing of the motor nerve, to augment contractile activity in muscles of intact mice. Both of these procedures are associated with changes in endogenous gene expression consistent with fiber type transformation. These procedures have previously been developed, tested and successfully used in wild type and transgenic animals under the supervision of Dr. Bassel-Duby at UT Southwestern. In addition, we will perform studies on isolated skeletal muscle function to study the isometric and isotonic contractile function of mouse skeletal muscle.
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