Urease Activity and Dental Caries in Children

脲酶活性与儿童龋齿

• 批准号: 6871943
• 负责人: EVANGELIA MOROU-BERMUDEZ
• 金额: $ 10.76万
• 依托单位: UNIVERSITY OF PUERTO RICO MED SCIENCES
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6871943
• 关键词: Puerto Rican; acidity /alkalinity; age difference; bacterial proteins; clinical research; dental caries; dental caries inhibitor; dental deposit removal; dental disorder diagnosis; dental plaque; dietary carbohydrates; disease /disorder proneness /risk; enzyme activity; gender difference; human subject; longitudinal human study; microorganism population study; nucleic acid hybridization; oral bacteria; patient oriented research; preschool child (1-5); saliva; single cell analysis; tissue resource /registry; urease

DESCRIPTION: The Candidate in this revised application for a K23 Award has graduate clinical training in Pediatric Dentistry and PhD level research training in Microbiology and in Cariology. The purpose of the requested Award is to provide the candidate with multidisciplinary training in patient-oriented research, which will help her design and conduct translational studies related to biological predictors of caries risk in children. The Career Development Plan of this proposal consists of a multidisciplinary program, which includes training in the responsible conduct of research, epidemiology, biostatistics and clinical research design, among others. The plan also includes a number of activities that will provide additional hands-on experience in clinical research and continuous training in molecular biology throughout the Award period. The Research component of this application consists of a combined clinical and basic research study, which aims to address the role bacterial urease activity in the development of dental caries in children. Bacterial urease activity in dental plaque generates ammonia, which is believed to inhibit the development of dental caries, and which can also influence the oral microbial ecology. If these relationships can be demonstrated, future studies could lead to the development of novel methods for caries risk assessment and prevention. The primary mentor is Dr. Augusto Elias, Professor and Director of the Research Office in the UPR School of Dentistry. Dr. Elias has extensive experience as a mentor, as a reference examiner in caries studies worldwide, and as the principal investigator of numerous corporate-sponsored dental clinical trials and prevalence studies in Puerto Rico. Dr. Elias will be joined in his role as a mentor by two co-mentors, Dr. Ronald Billings (University of Rochester) and Dr. Robert Burne (University of Florida) who will provide expert advice and technical support to the clinical and the basic science portion of the study, respectively. Dr. Erick Suarez, Professor in Biostatistics, will guide the candidate in study design methodology and in the analysis of the data.

描述：该修订后的K23奖项申请中的候选人接受了小儿牙科和博士学位研究培训微生物学和商cariology的临床培训。请求奖励的目的是为候选人提供以患者为导向的研究的多学科培训，这将有助于她的设计和进行与儿童龋齿风险的生物学预测因子有关的转化研究。 该提案的职业发展计划包括一个多学科计划，其中包括在负责任的研究，流行病学，生物统计学和临床​​研究设计方面进行培训。该计划还包括许多活动，这些活动将在整个奖励期间提供临床研究和分子生物学持续培训的额外动手经验。 该应用程序的研究组成部分由一项临床和基础研究组成，旨在解决细菌尿素释放酶活性在儿童牙科发展中的作用。牙菌斑中的细菌尿酶活性会产生氨，据信氨抑制龋齿的发展，并且也会影响口腔微生物生态学。如果可以证明这些关系，未来的研究可能会导致开发用于龋齿风险评估和预防的新方法。 主要导师是UPR牙科研究院教授兼主任Augusto Elias博士。 Elias博士在全球Caries研究的参考研究员中拥有丰富的经验，并且是波多黎各众多公司赞助的牙科临床试验和患病率研究的主要研究者。埃里亚斯博士将由两位联合会员罗纳德·比林斯（Ronald Billings）（罗切斯特大学）和罗伯特·伯恩（Robert Burne）博士（佛罗里达大学）担任导师，他们将分别为研究的临床和基础科学部分提供专家建议和技术支持。生物统计学教授埃里克·苏亚雷斯（Erick Suarez）博士将指导研究设计方法论和数据分析中的候选人。