Development of technology to maintain and monitor viable and functional tissue in longterm culture

开发在长期培养中维持和监测活组织和功能组织的技术

• 批准号: 2508484
• 金额: --
• 依托单位: University of Southampton
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2508484
• 关键词: Development; technology; maintain; monitor; viable

The process of discovery and development of new treatments is lengthy and costly, with a high rate of attrition between the preclinical and the clinical stages. This is mainly due to the inaccuracy of the methods and models used in the preclinical phase (traditional cell cultures and animal models). Thus, there is a need to investigate models that can recapitulate more precisely the physiological condition. In recent years, a new and promising approach has emerged: microphysiological systems, more specifically, organ-on-chip. This technology is becoming increasingly relevant for the study of disease states and to model organ functions and is already presenting promising results and breakthroughs. However, it still has some limitations due mainly to cell sourcing, and the still existing discrepancy with the in vivo situation. This report explores the different models used nowadays, their benefits and limitations, and presents a different approach that has not been widely investigated yet: the culture of whole tissue biopsies.

发现和开发新疗法的过程漫长而昂贵，临床前和临床阶段之间的损耗率很高。这主要是由于临床前阶段使用的方法和模型（传统的细胞培养和动物模型）不准确。因此，需要研究能够更精确地概括生理条件的模型。近年来，出现了一种新的和有前途的方法：微生理系统，更具体地说，器官芯片。这项技术对于疾病状态的研究和器官功能的建模越来越重要，并且已经呈现出有希望的结果和突破。然而，它仍然有一些局限性，主要是由于细胞来源，并仍然存在与体内情况的差异。本报告探讨了目前使用的不同模型，它们的优点和局限性，并提出了一种尚未被广泛研究的不同方法：全组织活检培养。