The Biogenesis of the Golgi Apparatus in T.Brucei

布氏高尔基体的生物起源

• 批准号: 6885034
• 负责人: CHRISTOPHER L DE GRAFFENRIED
• 金额: $ 4.4万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2007-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6885034
• 关键词: Golgi apparatus; RNA interference; Trypanosoma brucei; cell component structure /function; cell cycle; cell cycle proteins; electron microscopy; endoplasmic reticulum; fluorescence microscopy; fluorescent dye /probe; glycosyltransferase; intracellular transport; kinetosome; membrane biogenesis; postdoctoral investigator; protein protein interaction; three dimensional imaging /topography; transport proteins

DESCRIPTION (provided by applicant): The Golgi apparatus is responsible for post-translational processing of the proteins and lipids biosynthesized in the ER, and then delivering them to different locations within and outside the cell. Considering the importance of the Golgi in protein traffic, its accurate duplication and inheritance during cell division are carefully orchestrated. In mammalian cells the Golgi exists in a higher order structure that is known as the Golgi ribbon, making direct observation of the duplication event difficult. Simpler eukaryotes that contain one or just a few Golgi stacks have therefore been used recently as model systems to study this process. Recent work in the Warren lab using the parasite Trypanosoma brucei has provided evidence that the old Golgi provides components necessary for establishing the new organelle, suggesting that its duplication is a templated process. T. brucei will be used to determine the precise contributions of the old Golgi and the new ER exit site to the construction of a new, functional Golgi. The carriers responsible for transport between the two Golgi and the role of other organelles in positioning the new Golgi will also be investigated. Specific Aims: (1) To determine if Golgi matrix proteins are transferred to the new Golgi prior to a glycosyltransferase; (2) to identify the carriers that mediate transfer from the old Golgi to the new; (3) to identify the role of the basal body in the inheritance of the new Golgi; (4) to observe when the new Golgi becomes competent to process cargo: (5) and to determine the role of the 'extra' Golgi that form during the late cell cycle.

描述（由申请人提供）： 高尔基体负责在ER中进行的蛋白质和脂质生物合成的蛋白质后期加工，然后将它们输送到细胞内外的不同位置。考虑到高尔基体在蛋白质流量中的重要性，精心策划了细胞分裂过程中其准确的重复和继承。在哺乳动物的细胞中，高尔基体以高阶结构（称为高尔基丝带）存在，这使得对重复事件的直接观察变得困难。因此，最近已将包含一个或仅几个高尔基体堆栈的更简单的真核生物用作研究此过程的模型系统。 Brucei使用寄生虫锥虫的最新工作提供了证据，证明旧的高尔基人提供了建立新的细胞器所需的组件，这表明其重复是一个模板的过程。布雷西（T. brucei）将用于确定旧高尔基（Golgi）和新的ER出口站点的确切贡献，以构建新的功能性高尔基。还将研究负责两种高尔基体和其他细胞器在定位新高尔基体中运输的载体。具体目的：（1）确定在糖基转移酶之前是否将高尔基体基质蛋白转移到新的高尔基体中； （2）确定介导从旧高尔基体转移到新的载体； （3）确定基础在新高尔基的遗传中的作用； （4）观察新的高尔基人何时有能力处理货物：（5）并确定在晚期细胞周期中形成的“额外”高尔基体的作用。