Origins of Cardiac Neural Crest in the Chick Epiblast

描述（由申请人提供）： 预期的心脏神经嵴，一个有助于心脏大动脉的细胞群，在神经管阶段位于后脑的背侧。然而，没有资料是可利用的起源和状态的承诺推定的心脏嵴细胞在神经褶皱形成前的上胚层。本研究的目的是：1）确定鸡心脏神经嵴在上胚层中的位置，2）确定该细胞群在上胚层中的定型状态。初步结果表明，在原肠胚阶段4，心脏神经嵴前体尚未隔离从其他嵴沿着前后轴。我的下一个实验将旨在识别第4阶段和第9阶段之间的心脏嵴前体。由于心脏神经嵴，不像其他神经嵴，是致力于其命运的神经管阶段9，我假设，心脏嵴可能已经提交之前，神经管阶段。我将使用Dil标记，电穿孔，鹌鹑/小鸡移植和延时成像来解决上述目标。这一建议将进一步加深我们对心脏神经嵴形成的理解，并为心脏嵴诱导的分子表征铺平道路。

DESCRIPTION (provided by applicant): The prospective cardiac neural crest, a cell population that contributes to the great arteries of the heart, resides in the dorsal aspect of the hindbrain at neural tube stages. However, no information is available on the origin and state of commitment of presumptive cardiac crest cells in the epiblast before neural fold formation. This proposal aims to 1) identify the location of prospective cardiac neural crest in the epiblast in chick, 2) determine the state of commitment of this cell population in the epiblast. Preliminary results suggest that, at gastrula stage 4, cardiac neural crest precursors are not yet segregated from other crest along the anterior posterior axis. My next experiments will aim at identifying the cardiac crest precursors between stage 4 and stage 9. Because the cardiac neural crest, unlike other neural crest, is committed to its fate by neural tube stage 9, I hypothesize that the cardiac crest might already be committed before neural tube stage. I will use Dil labeling, electroporation, quail/chick grafts and time-lapse imaging to resolve the aims listed above. This proposal will further our understanding of the formation of cardiac neural crest and pave the way to a molecular characterization of the induction of the cardiac crest.