Linker cell migration in C. elegans

线虫中的连接细胞迁移

• 批准号: 6887073
• 负责人: MIHOKO KATO
• 金额: $ 4.83万
• 依托单位: CALIFORNIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-02 至 2008-04-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6887073
• 关键词: Caenorhabditis elegans; RNA interference; biomarker; cell migration; cellular polarity; developmental genetics; genetic library; green fluorescent proteins; helminth genetics; postdoctoral investigator; reproductive development

DESCRIPTION (provided by applicant): Migration has a vital function in forming the body plan of an organism and yet underlies the process of metastasis in cancer. Although the cell biology of migration has traditionally been studied in vitro, an in vivo system is required to relate this process to both development and disease. This study will investigate the migration of an individual cell in C. elegans, using both genetic and cell biological approaches, with the goal of understanding how genes function in a molecular pathway to ultimately regulate the cell biology of migration. Specifically, the migration of the linker cell (LC), which is the leader cell for gonad migration in the male, will be examined using three approaches. Molecular markers based on green fluorescent protein (GFP) will be developed to examine in live animals the LC, its surrounding extracellular matrix, and its intracellular polarity as it migrates. A screen using the RNAi library will be conducted to identify genes involved in LC migration. Finally, by using the molecular markers described above and building new tools, such as translational reporters, the function of selected genes and gene networks in regulating the cell biology of migration will be investigated.

描述（由申请人提供）： 迁移在形成有机体的身体计划中具有至关重要的功能，但却是癌症转移过程的基础。尽管传统上已经研究了迁移的细胞生物学，但需要一个体内系统才能将该过程与发育和疾病联系起来。这项研究将使用遗传和细胞生物学方法研究秀丽隐杆线虫中单个细胞的迁移，目的是了解基因在分子途径中的作用，以最终调节迁移的细胞生物学。具体而言，将使用三种方法检查接头细胞（LC）的迁移（LC），该连接器细胞（LC）是男性迁移的领导者。将开发基于绿色荧光蛋白（GFP）的分子标记物，以检查活动物中的LC，其周围的细胞外基质及其迁移时的细胞内极性。将进行使用RNAi库的屏幕，以识别参与LC迁移的基因。最后，通过使用上述分子标记并构建新工具，例如翻译记者，将研究选定的基因和基因网络在调节迁移细胞生物学中的功能。