Global analysis of gene regulation in the midline CNS

中枢神经系统基因调控的整体分析

• 批准号: 6943650
• 负责人: JOSEPH B KEARNEY
• 金额: $ 4.83万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-23 至 2007-07-22
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6943650
• 关键词: Drosophilidae; RNA interference; arthropod genetics; central nervous system; confocal scanning microscopy; developmental genetics; fluorescent in situ hybridization; functional /structural genomics; genetic mapping; genetic regulation; genetic screening; glia; high throughput technology; microarray technology; mutant; postdoctoral investigator; protein localization

DESCRIPTION (provided by applicant): Specialized midline precursors of the developing Drosophila embryo give rise to specific sets of neurons and gila, and serve as an excellent model system for the study of CNS development and function. Using available data, our lab has constructed a database of 297 putative midline-expressed genes. Since the exact expression patterns of a majority of these genes are unknown, a major goal of this proposal is to use high throughput RNA in situ hybridization to map the expression patterns of these genes during CNS midline development. This analysis will provide (1) a refined molecular map of the midline CNS and (2) serve as a reference for the analysis of midline CNS gene transcription. To determine which of these genes are involved in glial development, midline-glial-expressed genes will be functionally screened for glial phenotypes using RNA interference and available genetic mutants. For glial genes encoding transciption factors, in situ expression pattern screening and microarray analysis will be used to identify transcriptional changes in mutant genetic backgrounds. This analysis will provide a clearer understanding midline CNS development and the transcriptional pathways that regulate midline glial development and function.

描述（由申请人提供）：发育中的果蝇胚胎的专门中线前体产生特定的神经元和神经胶质细胞组，并作为研究CNS发育和功能的极好模型系统。利用现有的数据，我们的实验室已经构建了一个297个假定的中线表达基因的数据库。由于大多数这些基因的确切表达模式是未知的，这个建议的一个主要目标是使用高通量RNA原位杂交来绘制这些基因在CNS中线发育过程中的表达模式。该分析将提供（1）中线CNS的精确分子图谱和（2）作为中线CNS基因转录分析的参考。为了确定这些基因中的哪些参与神经胶质发育，将使用RNA干扰和可用的遗传突变体对中线神经胶质表达基因进行神经胶质表型的功能筛选。对于编码转录因子的神经胶质基因，将使用原位表达模式筛选和微阵列分析来鉴定突变遗传背景中的转录变化。这项分析将提供一个更清楚的了解中线中枢神经系统的发展和转录途径，调节中线胶质细胞的发展和功能。