Applying next generation structural biology methods to bacterial vaccine production (Ref: 3974)

将下一代结构生物学方法应用于细菌疫苗生产（参考号：3974）

• 批准号: 2578366
• 金额: --
• 依托单位: UNIVERSITY OF EXETER
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2578366
• 关键词: Applying; next; generation; structural; biology

This project will offer an exciting opportunity to learn upcoming structural biology methods, and to apply these to avaccinology project. The key methodological focus of the project will be micro electron diffraction (micro-ED), amethod of determining protein structures that will become a mainstream method over the next five years. The trainingfrom this PhD will make the student very competitive for industry and academic positions at the end of the project.The project will allow the student to learn methods for preparing samples, collecting data, and using this data todetermine protein structures, as well as complementary experimental and computational methods for understandingthe experimental data.The project will determine the structure of proteins from Coxiella burnetii, a human pathogen that also causeseconomic losses in cattle worldwide. The Harmer group are part of a consortium aiming to develop a polysaccharidebased vaccine against C. burnetii. We are determining the structures of important polysaccharide biosynthesis proteinsto aid the production of a recombinant vaccine. This project will contribute to this programme and offer opportunitiesto work with others in the consortium. Structures will also be interrogated using modelling techniques to determinewhether any proteins might act as drug targets.

这个项目将提供一个令人兴奋的机会，学习即将到来的结构生物学方法，并将其应用于禽流感项目。该项目的主要方法学重点将是微电子衍射（micro-ED），这是一种确定蛋白质结构的方法，将在未来五年内成为主流方法。该博士的培训将使学生在项目结束时在工业和学术职位上非常有竞争力。该项目将允许学生学习制备样品，收集数据和使用这些数据确定蛋白质结构的方法，以及理解实验数据的补充实验和计算方法。该项目将确定来自贝氏柯克斯体的蛋白质结构，a human人类pathogen病原that also也cause原因economic经济losses损失in cattle牛worldwide世界. Harmer研究小组是一个旨在开发一种基于多糖的抗C.伯内特氏菌我们正在确定重要的多糖生物合成蛋白质的结构，以帮助生产重组疫苗。该项目将有助于这一方案，并提供与联合体其他成员合作的机会。结构也将使用建模技术进行查询，以确定是否有任何蛋白质可能作为药物靶点。