Molecular characterisation of the genes and enzymes that plants use to produce DMSP

植物用于生产 DMSP 的基因和酶的分子表征

• 批准号: 2586084
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2586084
• 关键词: Molecular; characterisation; genes; enzymes; plants

Dimethylsulfoniopropionate (DMSP) is an anti-stress compound with key roles in global nutrient and sulfur cycling, signalling and climate. DMSP functions as an osmoprotectant, cryoprotectant, protectant against oxidative stress and a grazing deterrent in bacteria and marina algae. Only some plants have been shown to produce DMSP, including the saltmarsh cordgrass Spartina and tomato. The role of DMSP in these plants is unclear and, despiteknowing the biochemical pathway for DMSP synthesis, the key genes and enzymes involved in DMSP production remain undiscovered in plants.This project will use tomato as a model system and aims to unpick the molecular pathway for DMSP synthesis in plants and to understand why plants produce this compound. Enzymes involved in plant DMSP production have recently been identified and the enzymology of these gene products will be cloned and tested. Expression of the genes in plants grown under different conditions will be measured using qRT-PCR and mutant lines will be generated using CRISPR gene editing. The growth and physiology of these lines will be studied to determine if environmental factors affect their productivity and growth.

DMSP是一种抗应激化合物，在全球营养和硫循环、信号传导和气候中发挥关键作用。DMSP在细菌和海藻中起着植物保护剂、冷冻保护剂、抗氧化应激保护剂和放牧威慑剂的作用。只有一些植物被证明能产生DMSP，包括盐沼绳草Spartina和番茄。DMSP在这些植物中的作用尚不清楚，尽管知道DMSP合成的生化途径，但参与DMSP合成的关键基因和酶在植物中仍然未知，本项目将以番茄为模型系统，旨在揭示植物中DMSP合成的分子途径，并了解植物产生该化合物的原因。参与植物DMSP生产的酶最近已被鉴定，这些基因产物的酶学将被克隆和测试。将使用qRT-PCR测量在不同条件下生长的植物中基因的表达，并使用CRISPR基因编辑产生突变株系。将研究这些品系的生长和生理，以确定环境因素是否影响其生产力和生长。