Gene-edited potatoes with improved quality and health benefits

基因编辑马铃薯具有更高的质量和健康益处

• 批准号: 2588171
• 金额: --
• 依托单位: University of East Anglia
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2588171
• 关键词: Gene; edited; potatoes; improved; quality

Potatoes are a staple source of carbohydrates in many parts of the world, and it is a priority for biotechnology to create healthier potatoes that have improved post-harvest quality. Gene editing can effectively inactivate target genes to introduce desirable qualities in crops. For example, inactivating a starch branching enzyme can lead to more resistant starch in potato, which lowers glycaemic index and benefits gut health. However, inactivating target genes in the entire plant can in many cases lead to undesirable phenotypes and yield loss. It remains a challenge to deploy gene editing in a tissue-specific manner without transgenesis.The application of technology will require the profiling of small RNAs present in potato tubers during development and storage. Subsequent changes in starch and sugar metabolism of tubers from edited plants will be characterised using established biochemical analyses.

马铃薯是世界许多地区碳水化合物的主要来源，生物技术的一个优先事项是创造更健康的马铃薯，提高收获后的质量。基因编辑可以有效地修饰靶基因，从而在作物中引入所需的品质。例如，使淀粉分支酶失活可导致马铃薯中的抗性淀粉更多，从而降低血液指数并有益于肠道健康。然而，在整个植物中失活靶基因在许多情况下可导致不期望的表型和产量损失。在没有转基因的情况下以组织特异性方式部署基因编辑仍然是一个挑战。该技术的应用将需要分析马铃薯块茎在发育和储存过程中存在的小RNA。来自编辑植物的块茎的淀粉和糖代谢的后续变化将使用已建立的生物化学分析来表征。