Using fatberg waste as a feedstock to produce fragrance compounds in engineered E. coli

使用脂肪山废物作为原料在工程大肠杆菌中生产芳香化合物

• 批准号: 2589209
• 金额: --
• 依托单位: University of Edinburgh
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2589209
• 关键词: Using; fatberg; waste; feedstock; produce

The 'upcycling' of low-value material is a major focus of current research in biotechnology and has the potential to significantly reduce our society's reliance on fossil fuels by creating a circular economy.An emerging new approach to achieving this goal is the combined use of chemical catalysts and engineered microorganisms. This enables the use of 'non-natural' waste as a feedstock for biological processes, and dramatically expands the range of industrial compounds that can be produced from engineered cellular systems via fermentation. This also avoids the need to evolve new enzymes and strains capable of performing new-to-nature reactions; existing green chemical methods can instead be directly applied in vivo and interfaced with cellular metabolism.By merging synthetic biology and synthetic chemistry, this project will use fatberg waste from domestic sewers as a feedstock to produce flavour, fragrance and cosmetic compounds in engineered E. coli. Modern synthetic biology techniques will be used to construct new biosynthetic pathways (https://www.ed.ac.uk/biology/research/facilities/edinburgh-genome-foundry) and these will be interfaced with green chemical catalysts in vivo. Initial work will focus on the use of model feedstock mixtures before using real industry samples isolated from UK sewers.Overall, this project offers a unique opportunity to lead the development of a novel bioprocess to generate high value compounds from low value sewer waste to fuel the circular bioeconomy in the UK. This highly multidisciplinary project will be based in the Wallace Lab at the University of Edinburgh (http://wallacelab.bio.ed.ac.uk), a friendly and inclusive research team based at the Institute of Quantitative Biology, Biochemistry and Biotechnology (IQB3) in the School of Biological Sciences and the Centre for Synthetic and Systems Biology (SynthSys, https://www.ed.ac.uk/biology/synthsys). The lab offers a unique training environment for doctoral students to gain experience in a broad range of synthetic biology and synthetic chemistry techniques - including metabolic pathway design, DNA assembly, microbiology and protein biochemistry; in addition to organic synthesis, chemical biology and the analysis of microbial metabolites by HPLC, GC, NMR and LC-MS.This is a 4 year fully-funded IBioIC/BBSRC studentship in collaboration with Argent Energy and will involve a 3-/6-month industrial placement in addition to regular interactions with industry research scientists throughout the project. The student will join a cohort of students enrolled on the IBioIC CTP (https://www.ibioicctp.com) and will benefit from the wide range of additional training opportunities this provides. http://wallacelab.bio.ed.ac.uk

低价值材料的“升级循环”是当前生物技术研究的一个主要焦点，通过创建循环经济，它有可能显著降低我们社会对化石燃料的依赖。实现这一目标的一种新兴新方法是化学催化剂和工程微生物的联合使用。这使得使用“非天然”废物作为生物过程的原料成为可能，并大大扩大了可以通过发酵从工程细胞系统生产的工业化合物的范围。这也避免了需要进化新的酶和菌株，能够进行新的自然反应;现有的绿色化学方法可以直接应用于体内，并与细胞代谢相结合。通过融合合成生物学和合成化学，该项目将使用来自生活下水道的脂肪废物作为原料，在工程E.杆菌现代合成生物学技术将用于构建新的生物合成途径（https：//www.ed.ac.uk/biology/research/facilities/edinburgh-genome-foundry），这些途径将与体内绿色化学催化剂相结合。初步工作将集中在使用模型原料混合物之前，使用从英国下水道分离的真实的工业样品。总体而言，该项目提供了一个独特的机会，以引领开发一种新的生物工艺，从低价值的下水道废物中产生高价值的化合物，为英国的循环生物经济提供燃料。这个高度多学科的项目将基于爱丁堡大学的华莱士实验室（http：wallacelab.bio.ed.ac.uk），这是一个友好和包容的研究团队，位于生物科学学院的定量生物学，生物化学和生物技术研究所（IQB 3）和合成与系统生物学中心（SynthSys，https：//www.ed.ac.uk/biology/biology/biologysys）。该实验室为博士生提供了独特的培训环境，以获得广泛的合成生物学和合成化学技术的经验，包括代谢途径设计、DNA组装、微生物学和蛋白质生物化学;除了有机合成，化学生物学和通过HPLC，GC，NMR和LC-MS。这是一个与Argent Energy合作的为期4年的全额资助的IBioIC/BBSRC学生奖学金，除了在整个项目中与行业研究科学家进行定期互动外，还将涉及3-/6个月的工业实习。学生将加入一个学生在IBioIC CTP（https：//www.example.com）注册的队列，并将受益于广泛的额外的培训机会，这提供。www.ibioicctp.com http://wallacelab.bio.ed.ac.uk