The role of the ATM gene in familial breast cancer

ATM基因在家族性乳腺癌中的作用

• 批准号: 6825514
• 负责人: GEORGIA CHENEVIX-TRENCH
• 金额: $ 80.69万
• 依托单位: QUEENSLAND INSTITUTE OF MEDICAL RESEARCH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-07 至 2009-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6825514
• 关键词: breast neoplasm /cancer diagnosis; breast neoplasms; cancer risk; clinical research; family genetics; female; gene frequency; genetic polymorphism; genetic screening; genetic susceptibility; genotype; high performance liquid chromatography; human subject; loss of heterozygosity; neoplasm /cancer genetics; phenotype; protein structure function; tumor suppressor genes

DESCRIPTION (provided by applicant): The role of the ATM gene in breast cancer predisposition is controversial. Studies of carriers of ATM mutations have indicated that females have on average a 4-7 fold increased risk of breast cancer, but mutation analysis of the ATM gene in unselected breast cancer cases has failed to find a convincingly increased frequency compared with controls. However, we have shown that two specific pathogenic mutations of the ATM gene do occur in 4% of multiple-case breast cancer families, and confer high risks of breast cancer, equivalent to a risk to age 70 years of 60%. The broad aim of this proposal is to extend our finding by estimating the penetrance and frequency of breast cancer-causing ATM mutations by analysis of the gene in almost 3000 putative hereditary breast cancer families from North America and Australia. Our specific aims are to: 1) Perform mutation screening of ATM in the youngest affected female from 900 breast cancer families. 2) Identify putative ATM mutations among the variants found by analyses of the behaviour of these variants in tumors and cell lines from women who carry them. 3) Screen 2000 additional breast cancer families for putative ATM mutations defined in Aim 2. 4) Genotype the affected and unaffected family members of those individuals found to carry putative ATM mutations, and estimate the penetrance of these mutations. 5) Estimate the frequency of ATM mutations in defined groups of unselected population-based breast cancer cases. Our findings will have numerous clinical implications, including whether women in high-risk families should be routinely or selectively screened for ATM, as well as BRCA1 and BRCA2 mutations. In addition, knowledge of ATM mutation status will allow future assessment of many clinical issues, including genetic and environmental modifiers of penetrance, appropriate surveillance, disease severity, patients' response to different treatment regimens, and possible adverse reactions to radiotherapy. The results of this research may directly affect treatment decisions, and improve preventive monitoring in high-risk families.

描述（由申请人提供）：ATM基因在乳腺癌易感性中的作用是有争议的。对ATM突变携带者的研究表明，女性患乳腺癌的风险平均增加4-7倍，但对乳腺癌病例中ATM基因的突变分析未能发现与对照组相比有令人信服的增加频率。然而，我们已经表明，ATM基因的两个特定的致病突变确实发生在4%的多病例乳腺癌家族中，并赋予乳腺癌的高风险，相当于70岁的风险为60%。这项建议的主要目的是通过分析来自北美和澳大利亚的近3000个假定的遗传性乳腺癌家族的基因来估计导致乳腺癌的ATM突变的频率和频率，从而扩展我们的发现。我们的具体目标是：1）对900个乳腺癌家系中最年轻的受影响女性进行ATM突变筛查。2)通过分析携带这些变体的妇女的肿瘤和细胞系中这些变体的行为，在发现的变体中识别推定的ATM突变。3)筛选2000个额外的乳腺癌家族，以确定目标2中定义的推定ATM突变。4)对那些被发现携带推定的ATM突变的个体的受影响和未受影响的家庭成员进行基因分型，并估计这些突变的频率。5)估计ATM突变的频率，在确定的群体中，基于人群的乳腺癌病例。我们的研究结果将有许多临床意义，包括高危家庭的妇女是否应该进行常规或选择性ATM筛查，以及BRCA 1和BRCA 2突变。此外，ATM突变状态的知识将允许未来评估许多临床问题，包括遗传和环境修饰剂的转移，适当的监测，疾病的严重程度，患者对不同治疗方案的反应，以及可能对放疗的不良反应。这项研究的结果可能直接影响治疗决策，并改善高危家庭的预防性监测。