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Growing Three Dimensional Vascularized Structures to Mimic Natural Tissue

生长三维血管结构来模仿自然组织

基本信息

批准号：
2597548
负责人：
金额：
--
依托单位：
University of Cambridge
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2021
资助国家：
英国
起止时间：
2021 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2597548
关键词：
Growing Three Dimensional Vascularized Structures

项目摘要

The Medical Materials Group has extensive experience in the development of collagen scaffolds that mimic tissue structures. Collagens predominate in animal and human tissue and their role is two-fold: to provide a suitable mechanical support and to foster cell attachment and function. Freeze-drying followed by zero length cross linking provides a base technology for the production of 3D collagen environments and by controlling the chemistry of the process, it is possible to control the mechanical response and degradation properties. We have worked in the area of dermal tissues, cardiac tissues and nerve regeneration and are able to reproduce the architecture of natural tissue, providing space for cell colonization and for nutrient and oxygen transport. However, we also understand the importance of our three dimensional scaffold environments for the development of blood vessels which are key to tissue repair and regeneration. Through careful choice of cell type and culture conditions we have begun to reproduce vascularized tissue structures and these offer an important step further toward recapitulating the native tissues. We would propose to explore the fabrication of 3D collagen scaffolds using ice templating and characterize these using X-ray microtomography, scanning electron microscopy and mechanical testing. We will then use co-culture of appropriate biological cells to create a vascularized structure that mimics native tissue.

医疗材料集团在开发模拟组织结构的胶原蛋白支架方面拥有丰富的经验。胶原蛋白在动物和人类组织中占主导地位，其作用是双重的：提供合适的机械支持并促进细胞附着和功能。冷冻干燥后零长度交联提供了用于生产3D胶原环境的基础技术，并且通过控制该过程的化学，可以控制机械响应和降解特性。我们在皮肤组织、心脏组织和神经再生领域开展工作，能够复制天然组织的结构，为细胞定植以及营养和氧气运输提供空间。然而，我们也了解我们的三维支架环境对于血管发育的重要性，血管是组织修复和再生的关键。通过仔细选择细胞类型和培养条件，我们已经开始复制血管化的组织结构，这些提供了一个重要的步骤，进一步重演天然组织。我们建议探索使用冰模板制作3D胶原支架，并使用X射线显微断层扫描，扫描电子显微镜和机械测试来表征这些。然后，我们将使用适当的生物细胞的共培养来创建模拟天然组织的血管化结构。