Investigating the mechanism of the bacterial protection system MsrP/Q using EPR spectroscopy

使用 EPR 光谱研究细菌保护系统 MsrP/Q 的机制

• 批准号: 2598163
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2598163
• 关键词: Investigating; mechanism; bacterial; protection; system

The methionine sulfoxide reductases MsrP and MsrQ play an important role in protecting bacteria, including major human pathogens, against reactive oxygen and chlorine species. However, their mechanism of action is not well understood. In this project, we will employ electron paramagnetic resonance (EPR) techniques to identify and understand the role of radical species in these complex reactions. Moreover, the novel methodology developed to study the MsrP/Q system is anticipated to be more generally applicable to redox-active enzymes.

蛋氨酸亚砜还原酶MsrP和MsrQ在保护细菌（包括主要的人类病原体）免受活性氧和氯的侵害方面发挥着重要作用。然而，它们的作用机制尚不清楚。在这个项目中，我们将采用电子顺磁共振（EPR）技术来识别和了解自由基在这些复杂反应中的作用。此外，用于研究MsrP/Q系统的新方法预计将更广泛地适用于氧化还原酶。