Core--Molecular biology, immunocytochemistry and imaging

描述(由申请人提供) 核心将为分子生物学、免疫细胞化学的应用提供设施 和成像技术。为促进这些目标，核心将提供：1)技术援助 用于组织制备和细胞培养，用于分子生物学技术和 免疫组织化学，2)提供专业生化或 免疫组织化学技术，3)容易获得专门的设备，4)成本 通过集中的设备和设施提高效率，5)在 不同的调查员。 核心提供的服务、指导和专门设备将发挥关键作用 在以高效和有效的方式成功完成研究项目方面发挥作用。 分子生物学、免疫细胞化学和成像核心的具体目标是： 1.为研究人员在细胞培养、基因转移等方面提供帮助和便利。 细胞和组织中的表达，并帮助Northern和Western对基因表达进行量化 印迹分析并用适当的方法估计转移基因的功能 生化分析。 2.提供用于组织化学和荧光检测的设施 细胞和组织中的报告蛋白和转基因产物。 3.为细胞类型和神经的免疫荧光测定提供帮助 使用多种抗体终止特定的转基因表达。 4.积极配合PPG调查人员设计和执行实验 与细胞内阳离子动态平衡、膜电位和 刺激-反应耦合过程中细胞的氧化还原状态。这通常会涉及到程序 显微视频数字图像分析系统，以及共焦显微镜。 5.在分子生物学技术方面提供便利和协助，以评估 Northern分析和RT-PCR检测基因表达水平。 6.核心的活动将以促进新技术的发展为目标 以及对重点问题的合作调查。

DESCRIPTION (provided by applicant) The Core will provide facilities for the application of molecular biology, immunocytochemical and imaging techniques. To facilitate these goals the core will provide: 1) technical assistance for tissue preparation and cell culture for molecular biology techniques and immunohistochemistry, 2) provide technical assistance in specialized biochemical or immunohistochemical techniques, 3) ready access to specialized equipment, 4) cost effectiveness through centralized equipment and facilities, 5) sharing of knowledge between different investigators. The services, instruction and specialized equipment provided by the Core will play a critical role in the successful completion of the research projects in an efficient and effective manner. The specific aims of the Molecular Biology, Immunocytochemistry and Imaging Core are: 1. To provide assistance and facilities to investigators for cell culture, gene transfer in cultured cells and in tissues, and help in the quantification of gene expression by Northern and Western blot analysis and to estimate the functionality of the transferred gene using appropriate biochemical assays. 2. To provide facilities for histochemical and fluorescence applications for the detection of reporter proteins and transgene products in cells and tissues. 3. To provide assistance in immunofluorescence determinations of cell type and nerve terminals specific transgene expression using multiple antibodies. 4. To actively collaborate with the PPG investigators in designing and executing experiments pertaining to measurements of intracellular cation homeostasis, membrane potential and redox state of the cell during stimulus-response coupling. This will generally involve procedures of microscopic video digital image analysis system, and confocal microscopy. 5. To provide facilities and assistance in molecular biology techniques for the estimation of mRNA levels by Northern analysis and RT-PCR. 6. The activities of the core will be directed towards fostered development of new technology as well as collaborative investigations of focused problems.