Core--Molecular biology, immunocytochemistry and imaging
Core--Molecular biology, immunocytochemistry and imaging
批准号:
6704855
负责人:
RAMESH C BHALLA
金额:
$4.83万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2003
资助国家:
美国
项目状态:
已结题
起止时间:
2003-01-21 至 2007-12-31
中文摘要
描述(由申请人提供)
英文摘要
DESCRIPTION (provided by applicant)
The Core will provide facilities for the application of molecular biology, immunocytochemical
and imaging techniques. To facilitate these goals the core will provide: 1) technical assistance
for tissue preparation and cell culture for molecular biology techniques and
immunohistochemistry, 2) provide technical assistance in specialized biochemical or
immunohistochemical techniques, 3) ready access to specialized equipment, 4) cost
effectiveness through centralized equipment and facilities, 5) sharing of knowledge between
different investigators.
The services, instruction and specialized equipment provided by the Core will play a critical
role in the successful completion of the research projects in an efficient and effective manner.
The specific aims of the Molecular Biology, Immunocytochemistry and Imaging Core are:
1. To provide assistance and facilities to investigators for cell culture, gene transfer in cultured
cells and in tissues, and help in the quantification of gene expression by Northern and Western
blot analysis and to estimate the functionality of the transferred gene using appropriate
biochemical assays.
2. To provide facilities for histochemical and fluorescence applications for the detection of
reporter proteins and transgene products in cells and tissues.
3. To provide assistance in immunofluorescence determinations of cell type and nerve
terminals specific transgene expression using multiple antibodies.
4. To actively collaborate with the PPG investigators in designing and executing experiments
pertaining to measurements of intracellular cation homeostasis, membrane potential and
redox state of the cell during stimulus-response coupling. This will generally involve procedures
of microscopic video digital image analysis system, and confocal microscopy.
5. To provide facilities and assistance in molecular biology techniques for the estimation of
mRNA levels by Northern analysis and RT-PCR.
6. The activities of the core will be directed towards fostered development of new technology
as well as collaborative investigations of focused problems.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
CORE--IMAGE PROCESSING
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批准号:6564798
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项目类别:
-
资助金额:$23.33万
-
财政年份:2002
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负责人:RAMESH C BHALLA
-
依托单位:
CORE--IMAGE PROCESSING
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批准号:6415225
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项目类别:
-
资助金额:$23.33万
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财政年份:2001
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负责人:RAMESH C BHALLA
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依托单位:
MECHANISMS OF VASCULAR WALL THICKENING OF AGED RATS
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批准号:6287558
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项目类别:
-
资助金额:$7.35万
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财政年份:2001
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负责人:RAMESH C BHALLA
-
依托单位:
CORE--IMAGE PROCESSING
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批准号:6302102
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项目类别:
-
资助金额:$19.28万
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财政年份:2000
-
负责人:RAMESH C BHALLA
-
依托单位:
CORE--IMAGE PROCESSING
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批准号:6109373
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项目类别:
-
资助金额:$19.28万
-
财政年份:1999
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负责人:RAMESH C BHALLA
-
依托单位:
CORE--IMAGE PROCESSING
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批准号:6272505
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项目类别:
-
资助金额:$18.15万
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财政年份:1998
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负责人:RAMESH C BHALLA
-
依托单位:
MECHANISMS OF ARTERIAL SUPERSENSITIVITY IN SHR
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批准号:3349816
-
项目类别:
-
资助金额:$12.57万
-
财政年份:1985
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负责人:RAMESH C BHALLA
-
依托单位:
MECHANISMS OF ARTERIAL SUPERSENSITIVITY IN SHR
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批准号:3349814
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项目类别:
-
资助金额:$11.41万
-
财政年份:1985
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负责人:RAMESH C BHALLA
-
依托单位:
MECHANISMS OF ARTERIAL SUPERSENSITIVITY IN SHR
-
批准号:3349810
-
项目类别:
-
资助金额:$13.18万
-
财政年份:1985
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负责人:RAMESH C BHALLA
-
依托单位:
MECHANISMS OF ARTERIAL SUPERSENSITIVITY IN SHR
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批准号:3349813
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项目类别:
-
资助金额:$12.18万
-
财政年份:1985
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负责人:RAMESH C BHALLA
-
依托单位:
MECHANISMS OF ARTERIAL SUPERSENSITIVITY IN SHR
-
批准号:3349815
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项目类别:
-
资助金额:$12.72万
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财政年份:1985
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负责人:RAMESH C BHALLA
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依托单位:
CALCIUM/IONS AND CAMP ROLE IN CAUSATION OF HYPERTENSION
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批准号:3335722
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项目类别:
-
资助金额:$12.28万
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财政年份:1979
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负责人:RAMESH C BHALLA
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依托单位:
FEASIBILITY STUDY--CA HOMEOSTASIS AND MOTILITY OF VISCERAL SMOOTH MUSCLE IN SHR
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批准号:3940776
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:RAMESH C BHALLA
-
依托单位:
Core--Molecular biology, immunocytochemistry and imaging
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批准号:7569176
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项目类别:
-
资助金额:$7.68万
-
财政年份:--
-
负责人:RAMESH C BHALLA
-
依托单位:
FEASIBILITY STUDY--CA HOMEOSTASIS AND MOTILITY OF VISCERAL SMOOTH MUSCLE IN SHR
-
批准号:3964610
-
项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:RAMESH C BHALLA
-
依托单位:
FEASIBILITY STUDY--CA HOMEOSTASIS AND MOTILITY OF VISCERAL SMOOTH MUSCLE IN SHR
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批准号:3917868
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项目类别:
-
资助金额:$0.0万
-
财政年份:--
-
负责人:RAMESH C BHALLA
-
依托单位:
FEASIBILITY STUDY--CA HOMEOSTASIS AND MOTILITY OF VISCERAL SMOOTH MUSCLE IN SHR
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批准号:4689826
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项目类别:
-
资助金额:$0.0万
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财政年份:--
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负责人:RAMESH C BHALLA
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依托单位:
海外基金