Core--Molecular biology, immunocytochemistry and imaging

核心--分子生物学、免疫细胞化学和成像

• 批准号: 6704855
• 负责人: RAMESH C BHALLA
• 金额: $ 4.83万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-01-21 至 2007-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6704855
• 关键词: bioimaging /biomedical imaging; biomedical facility; confocal scanning microscopy; digital imaging; fluorescent dye /probe; histology; immunocytochemistry; immunofluorescence technique; microscopy; northern blottings; tissue /cell culture; transfection; video microscopy; western blottings

DESCRIPTION (provided by applicant) The Core will provide facilities for the application of molecular biology, immunocytochemical and imaging techniques. To facilitate these goals the core will provide: 1) technical assistance for tissue preparation and cell culture for molecular biology techniques and immunohistochemistry, 2) provide technical assistance in specialized biochemical or immunohistochemical techniques, 3) ready access to specialized equipment, 4) cost effectiveness through centralized equipment and facilities, 5) sharing of knowledge between different investigators. The services, instruction and specialized equipment provided by the Core will play a critical role in the successful completion of the research projects in an efficient and effective manner. The specific aims of the Molecular Biology, Immunocytochemistry and Imaging Core are: 1. To provide assistance and facilities to investigators for cell culture, gene transfer in cultured cells and in tissues, and help in the quantification of gene expression by Northern and Western blot analysis and to estimate the functionality of the transferred gene using appropriate biochemical assays. 2. To provide facilities for histochemical and fluorescence applications for the detection of reporter proteins and transgene products in cells and tissues. 3. To provide assistance in immunofluorescence determinations of cell type and nerve terminals specific transgene expression using multiple antibodies. 4. To actively collaborate with the PPG investigators in designing and executing experiments pertaining to measurements of intracellular cation homeostasis, membrane potential and redox state of the cell during stimulus-response coupling. This will generally involve procedures of microscopic video digital image analysis system, and confocal microscopy. 5. To provide facilities and assistance in molecular biology techniques for the estimation of mRNA levels by Northern analysis and RT-PCR. 6. The activities of the core will be directed towards fostered development of new technology as well as collaborative investigations of focused problems.

描述（由申请人提供） 该核心将为分子生物学、免疫细胞化学的应用提供设施 和成像技术。为了实现这些目标，核心将提供：1）技术援助 用于组织制备和细胞培养，用于分子生物学技术和 免疫组织化学，2) 提供专业生化或免疫组化方面的技术援助 免疫组织化学技术，3) 易于使用的专用设备，4) 成本 通过集中设备和设施提高效率，5) 之间的知识共享 不同的调查员。 核心提供的服务、指导和专业设备将发挥关键作用 以高效和有效的方式成功完成研究项目的作用。 分子生物学、免疫细胞化学和成像核心的具体目标是： 1. 为研究者提供细胞培养、培养物基因转移等方面的协助和便利 细胞和组织中，并有助于北方和西方基因表达的量化 印迹分析并使用适当的方法估计转移基因的功能 生化测定。 2. 提供组织化学和荧光应用的检测设施 细胞和组织中的报告蛋白和转基因产物。 3. 为细胞类型和神经的免疫荧光测定提供帮助 使用多种抗体终止特异性转基因表达。 4. 积极配合PPG研究人员设计和执行实验 关于细胞内阳离子稳态、膜电位和 刺激-反应耦合过程中细胞的氧化还原状态。这一般都会涉及一些程序 显微视频数字图像分析系统和共焦显微镜。 5. 为分子生物学技术的估计提供设施和帮助 通过 Northern 分析和 RT-PCR 检测 mRNA 水平。 6. 核心的活动将致力于促进新技术的发展 以及针对重点问题的协作调查。