Core--Molecular biology, immunocytochemistry and imaging

描述（由申请人提供） 该中心将为分子生物学、免疫细胞化学 和成像技术。为促进实现这些目标，核心小组将提供： 用于分子生物学技术的组织制备和细胞培养， 免疫组织化学，2）提供专门的生物化学或 免疫组织化学技术，3）易于获得专门设备，4）成本 通过集中的设备和设施提高效率，5） 不同的调查员 核心小组提供的服务、指导和专门设备将发挥关键作用， 以高效和有效的方式成功完成研究项目。 分子生物学，免疫细胞化学和成像核心的具体目标是： 1.为研究者进行细胞培养、培养细胞中的基因转移提供协助和便利。 在细胞和组织中，并帮助通过北方和西方的基因表达的定量 印迹分析，并使用适当的方法评估转移基因的功能性。 生化分析 2.为组织化学和荧光应用提供设施，用于检测 细胞和组织中的报告蛋白和转基因产物。 3.为细胞类型和神经的免疫荧光测定提供帮助 使用多种抗体终止特异性转基因表达。 4.积极与PPG研究者合作设计和执行实验 与细胞内阳离子稳态、膜电位和 在刺激-反应耦合期间细胞的氧化还原状态。这通常会涉及程序 显微视频数字图像分析系统和共聚焦显微镜。 5.提供分子生物学技术方面的设施和协助， 通过北方分析和RT-PCR检测mRNA水平。 6.该核心的活动将致力于促进新技术的发展 以及对重点问题的合作调查。

DESCRIPTION (provided by applicant) The Core will provide facilities for the application of molecular biology, immunocytochemical and imaging techniques. To facilitate these goals the core will provide: 1) technical assistance for tissue preparation and cell culture for molecular biology techniques and immunohistochemistry, 2) provide technical assistance in specialized biochemical or immunohistochemical techniques, 3) ready access to specialized equipment, 4) cost effectiveness through centralized equipment and facilities, 5) sharing of knowledge between different investigators. The services, instruction and specialized equipment provided by the Core will play a critical role in the successful completion of the research projects in an efficient and effective manner. The specific aims of the Molecular Biology, Immunocytochemistry and Imaging Core are: 1. To provide assistance and facilities to investigators for cell culture, gene transfer in cultured cells and in tissues, and help in the quantification of gene expression by Northern and Western blot analysis and to estimate the functionality of the transferred gene using appropriate biochemical assays. 2. To provide facilities for histochemical and fluorescence applications for the detection of reporter proteins and transgene products in cells and tissues. 3. To provide assistance in immunofluorescence determinations of cell type and nerve terminals specific transgene expression using multiple antibodies. 4. To actively collaborate with the PPG investigators in designing and executing experiments pertaining to measurements of intracellular cation homeostasis, membrane potential and redox state of the cell during stimulus-response coupling. This will generally involve procedures of microscopic video digital image analysis system, and confocal microscopy. 5. To provide facilities and assistance in molecular biology techniques for the estimation of mRNA levels by Northern analysis and RT-PCR. 6. The activities of the core will be directed towards fostered development of new technology as well as collaborative investigations of focused problems.