Generation of DNA aptamers to detect and differentiate between latent and active tuberculosis

生成 DNA 适体以检测和区分潜伏性结核病和活动性结核病

• 批准号: 2599262
• 金额: --
• 依托单位: Newcastle University
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2599262
• 关键词: Generation; DNA; aptamers; detect; differentiate

Tuberculosis (TB) is the leading cause of mortality from an infectious disease worldwide, with one third of humans already infected with Mycobacterium tuberculosis (Mtb), either in the active or latent form, representing a huge reservoir of disease. The current landscape of TB is characterised by; a lack of efficient diagnostic methods at the Point-of-Need, increasing appearance of multidrug resistant strains, ineffective therapeutic coverage and the lack of a universal vaccine. This project will improve diagnostic provision by developing novel recognition molecules - DNA-aptamers - that bind to unique biomarkers in the Mtb membrane, differentiating between active and latent TB infections.

结核病是世界范围内导致死亡的一种传染病，三分之一的人已经感染了结核分枝杆菌（Mtb），无论是活动性的还是潜伏性的，这是一个巨大的疾病库。当前结核病形势的特点是：在需求点缺乏有效的诊断方法，越来越多的耐多药菌株出现，无效的治疗覆盖以及缺乏通用疫苗。该项目将通过开发新的识别分子——dna适体——来改善诊断提供，这种分子与结核分枝杆菌膜上的独特生物标志物结合，从而区分活动性和潜伏性结核感染。