Development of an intradermal or inhaled DNA vaccine for COVID-19 and beyond

核酸疫苗的皮内和吸入递送在临床上是有效的、安全的，并可在全球范围内部署，以对抗多种复杂疾病。该项目旨在结合物理和基于肽的递送系统，以提高这些技术在我们的联盟（由Innovate-UK资助）目前正在开发的COVID-19疫苗（“covid”）中的功效和应用。该博士将在离体组织模型中制定和测试以皮肤和肺部为重点的疫苗递送的组合系统，然后转移到啮齿动物模型，检查病毒中和，T细胞反应和病毒攻击动物的保护。优化和演示的平台技术将对核酸疫苗方法（包括DNA、mRNA和寡核苷酸方法）的未来应用产生广泛影响。博士学位还将直接帮助将抗体和肽工程技术转化为针对多种复杂疾病的快速部署和安全的免疫系统。

Intradermal and inhaled delivery of nucleic acid vaccines could be clinically effective, safe and globally-deployable against multiple complex diseases. This project will aim to combine physical and peptide-based delivery systems to enhance the efficacy and application of such technologies to the COVID-19 vaccine ('Covidity') presently under-development by our consortium (funded by Innovate-UK). This PhD will formulate and test combined systems for skin and lung-focused vaccine delivery in ex vivo tissue models, then move to rodent models, examining virus neutralisation, T cell responses and protection in virally challenged animals. The platform technology optimised and demonstrated will have wide ranging impact on future applications of nucleic acid vaccine approaches (including DNA, mRNA and oligo-based methods). The PhD will also directly aid attempts to translate antibody and peptide-engineering knowhow to generate a rapidly deployable and safe immunisation system for multiple complex diseases.