CORE--MICROSCOPY AND IMAGING

北卡罗来纳大学莱恩伯格综合癌症中心的显微镜和成像设施在一个管理结构中结合了两个相关的核心职能：电子显微镜和光学显微镜。Jack Griffith博士指导电子显微镜部分，Robert Bagnell博士指导位于北卡罗来纳大学-CH显微镜服务实验室的新的光学显微镜服务部分。25年来，电子显微镜(EM)设备一直是北卡罗来纳大学LCCC的高效核心设备，专注于DNA和RNA分子及其与蛋白质的络合物的可视化。EM设施提供了一系列不断发展的新方法，包括全低温EM和全数字暗室工作。光学显微镜服务核心(LM)为癌症中心成员提供最先进的光学显微镜，在其使用和解释方面提供专家帮助，并在免疫方法、图像方面提供咨询 解释和测量。活细胞和高通量活细胞显微镜的进一步发展有望在下一个五年计划中实现。联合设施的整体协调是通过癌症中心和基础科学核心副主任William Marzluff博士进行的。然而，这两个组件是独立运行的。癌症中心管理电子显微镜部分，病理和实验室医学部管理显微镜服务实验室。

The UNC Lineberger Comprehensive Cancer Center Microscopy and Imaging facilities combine in one administrative structure two related core functions: electron microscopy and light microscopy. Dr. Jack Griffith directs the Electron Microscopy component, and Dr. Robert Bagnell directs the new Light Microscopy Services component housed in the UNC-CH Microscopy Services Laboratory. The Electron Microscopy (EM) facility has been a highly productive UNC LCCC Core Facility for over 25 years, focusing on the visualization of DNA and RNA molecules and their complexes with proteins. The EM facility makes available an evolving battery of new methods including full cryo EM and fully digital darkroom work. The Light Microscopy Services Core (LM) provides Cancer Center members with access to state-of-the-art light microscopy, expert assistance in its utilization and interpretation, and consultation in immuno methods, image interpretation and measurement. Further development of live cell and high throughput live cell microscopy is anticipated in the next five-year plan. Overall coordination of the combined facility is through the Cancer Center and Dr. William Marzluff, Associate Director for Basic Science Cores. The two components, however, operate independently. The Cancer Center administers the Electron Microscopy component, and the Department of Pathology and Laboratory Medicine administers the Microscopy Services Laboratory.