CORE--CELL AND IMAGING

该核心将协调和提供：1.人类志愿者PMN；2.批量的人类内皮细胞系(肺微血管hMVEC)；3.成像和光学实验设施(FRET、3D重建、多通道成像和细胞术)；以及4.有效使用易腐烂的标记一抗和二抗库存。目前，Core配备了两台数字去卷积显微镜和Beckman Coulter流式细胞仪以及全组织培养能力。这两个示波器都配备了在多达4个颜色通道中执行实时或固定细胞3D成像。该中心的工作人员在原代人体细胞培养和各种成像方法的各个方面都具有丰富的经验。核心将积极进入未来(2-4年)，1.将数据与国家数据库(主要是地球观测组织项目)联系起来，2.在需要之前扩大自己的分析方法库，3.与TCG内的其他核心分析工作(核磁共振、质谱学、微阵列)建立联系。

This core will coordinate and provide: 1. human volunteer PMNs; 2. batches of human endothelial cell lines (pulmonary microvascular hMVEC); 3. imaging and optical experiment facility (FRET, 3D reconstruction, multichannel imaging and cytometry); and 4. efficient use of a perishable inventory of labeled primary and secondary antibodies. At present the Core is equipped with two digital deconvolving microscopes and Beckman Coulter flow cytometer and full tissue culture capabilities. Both scopes are equipped to perform live or fixed cell 3D imaging in upto 4 color channels. The Core is staffed by personnel who are highly experienced in all aspects of primary human cell culture and various imaging methodologies. The core will actively move into the future (2-4 years), 1. by linking data to national databases (primarily the GEO project) and 2. expanding its own repertoire of analytical methods before the need arises, 3. Linking to other core analytical efforts (NMR, MS, microarrays) within the TCG.