CORE--CELL AND IMAGING

核心——细胞与成像

• 批准号: 6973946
• 负责人: ANIRBAN BANERJEE
• 金额: $ 18.53万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6973946
• 关键词: antibody; bioimaging /biomedical imaging; biomedical facility; cell line; chemical registry /resource; data management; digital imaging; flow cytometry; fluorescence resonance energy transfer; human genetic material tag; human subject; leukocytes; molecular /cellular imaging; three dimensional imaging /topography; tissue /cell culture

This core will coordinate and provide: 1. human volunteer PMNs; 2. batches of human endothelial cell lines (pulmonary microvascular hMVEC); 3. imaging and optical experiment facility (FRET, 3D reconstruction, multichannel imaging and cytometry); and 4. efficient use of a perishable inventory of labeled primary and secondary antibodies. At present the Core is equipped with two digital deconvolving microscopes and Beckman Coulter flow cytometer and full tissue culture capabilities. Both scopes are equipped to perform live or fixed cell 3D imaging in upto 4 color channels. The Core is staffed by personnel who are highly experienced in all aspects of primary human cell culture and various imaging methodologies. The core will actively move into the future (2-4 years), 1. by linking data to national databases (primarily the GEO project) and 2. expanding its own repertoire of analytical methods before the need arises, 3. Linking to other core analytical efforts (NMR, MS, microarrays) within the TCG.

该核心将协调并提供： 1. 人类志愿者 PMN； 2. 批次人内皮细胞系（肺微血管hMVEC）； 3. 成像和光学实验设施（FRET、3D重建、多通道成像和细胞计数）； 4. 有效利用易腐烂的标记一抗和二抗库存。目前，Core 配备了两台数字解卷积显微镜和贝克曼库尔特流式细胞仪以及完整的组织培养功能。两款示波器均可在多达 4 个颜色通道中执行实时或固定细胞 3D 成像。核心人员在原代人类细胞培养和各种成像方法的各个方面都具有丰富的经验。该核心将积极走向未来（2-4年），1.通过将数据链接到国家数据库（主要是GEO项目），2.在需要之前扩展其自己的分析方法库，3.链接到TCG内的其他核心分析工作（NMR、MS、微阵列）。