Perkin Elmer Ultraview Spinning Disc Confocal Microscope

Perkin Elmer Ultraview 转盘共焦显微镜

• 批准号: 6730980
• 负责人: ZENA WERB
• 金额: $ 34.48万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2006-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6730980
• 关键词: bioimaging /biomedical imaging; biomedical equipment purchase; confocal scanning microscopy

DESCRIPTION (provided by applicant): This is a request for funds to purchase a Perkin Elmer Ultraview RS-3 spinning disc confocal microscope system, with a Leica DM IRE2 motorized inverted microscope. The instrument will be housed in the Department of Anatomy, University of California, San Francisco. This multi-user facility will provide access for the user group to a three laser optical imaging system for rapid, real-time, three-color fluorescent imaging primarily for living cells, embryos and organoids, and intact live animal over extended periods of time. There is a critical need for instruments for live cell and animal imaging as functional proteomic approaches with green and other color fluorescent protein-tagged molecules, quantum microdots and other fluorescent -tagged reagents become the benchmark for high quality research. Currently there are a number of conventional confocal and multiphoton microscopes on the University of California, San Francisco that have the depth of imaging and several have three color capability, but they are not appropriate for these uses. The important aspects of this system are (a) not imaging depth, but imaging speed, which is needed for rapid cell movements, (b) reliability, (c) ease of use, (d) automated imaging of large areas of the sample at one time so that stochastic events can be caught, (e) very low photobleaching and phototoxicity over extended periods of imaging (20 min to 3 days) and (f) lower cost of use and maintenance. No comparable instrument exists. The core user group of six investigators has more than 14 grants from the National Institutes of Health on the Parnassus campus of University of California, San Francisco and has an impressive track record of accomplishments in a range of fields. As a part of a campus wide imaging core, on a time available basis the microscope will be made available to other users. The acquisition of the requested instrument will allow the individual users access to state-of-the-art live cell and animal imaging technology and thus enhance the productivity of their individual grants.

描述（由申请人提供）：这是一份购买Perkin Elmer Ultraview RS-3旋转圆盘共聚焦显微镜系统的资金申请，配有Leica DM IRE 2电动倒置显微镜。该仪器将被安置在弗朗西斯科加州大学解剖学系。该多用户设施将为用户组提供三激光光学成像系统，用于快速，实时，三色荧光成像，主要用于活细胞，胚胎和类器官，以及长时间的完整活动物。对于用于活细胞和动物成像的仪器存在关键需求，因为具有绿色和其它颜色荧光蛋白标记的分子、量子微粒和其它荧光标记的试剂的功能蛋白质组学方法成为高质量研究的基准。目前，加州大学旧金山分校弗朗西斯科有许多传统的共焦和多光子显微镜，它们具有成像深度，有几种具有三色能力，但它们不适合这些用途。该系统的重要方面是（a）不是成像深度，而是成像速度，这是快速细胞移动所需的，（B）可靠性，（c）易用性，（d）一次对样品的大面积自动成像，以便可以捕获随机事件，（e）在延长的成像时间段（20分钟至3天）内非常低的光漂白和光毒性，以及（f）较低的使用和维护成本。没有类似的文书。 由六名研究人员组成的核心用户组获得了加州大学弗朗西斯科分校帕纳萨斯校区美国国立卫生研究院的14多项赠款，并在一系列领域取得了令人印象深刻的成就。作为校园范围内成像核心的一部分，在可用时间的基础上，显微镜将提供给其他用户。获得所要求的仪器将使个人用户能够获得最先进的活细胞和动物成像技术，从而提高其个人赠款的生产力。