Blood and Tissue Specimen Core Facility

纽约哥伦比亚大学急性卒中翻译研究合作专门项目(NYCC-SPOTRIAS)的核心D(血液和组织核心)项目的目标是提供集中的、专业的样本处理管理，由实验室技术员在临床病理学家实验室主任的监督下在中央设施进行实验室测试，并在受控条件下为DMA和可用于重复和额外测试的标本等份提供长期存储的场所。这些职能将由哥伦比亚大学高级实验室医学中心(CAME)执行。据估计，在SPOTRIAS项目的整个过程中，将有680次标本收集，导致近7,000份等分需要处理、测试 和/或存储。采集后，标本将立即被送往CAME进行处理、等量测定和DMA提取。经过处理后，标本信息将被输入到Freezerworks标本管理软件中，并生成条形码标签并放置在标本上。由于标本被放置在零下80摄氏度的冷冻仓库中，试管将被条形码扫描，并将创建标本地图用于库存和检索目的。为了确保这些标本的安全，凯姆将在有二氧化碳备份的紧急电力电路上使用受监控的冰柜。同样，DNA也会 以类似的方式提取、等分并准备储存。Calm将对检索到的等分进行hs-CRP、IL6和TNF测试。所有测试将一式两份进行，并采用适当的质量控制程序，使用商业控制和复制样本；实验室还将订阅可用的熟练程度计划。CAMPE工作人员将监督将样本运送到医院实验室进行CK、肝脏轮廓和其他必要的测试；CAMPE工作人员还将检索这些测试的结果，并将其输入计算机数据库，以便传输给数据管理人员。

The aims of the Core D (Blood and Tissue Core) project of the New York Columbia Collaborative Specialized Program on Translational Research In Acute Stroke (NYCC-SPOTRIAS) are to provide centralized, professional management of specimen processing, to perform laboratory testing in a central facility by a laboratory technologist under the supervision of a Clinical Pathologist laboratory director, and to provide a place for long term storage under controlled conditions for DMA and specimen aliquots that can be used for repeat and additional testing. These functions will be performed by the Center for Advanced Laboratory Medicine (CALM) at Columbia University. It is estimated that over the course of the SPOTRIAS project, there will be 680 specimen collection episodes resulting in nearly 7,000 aliquots to be processed, tested and/or stored. Immediately after collection, specimens will be sent to CALM for processing, aliquotting, and DMA extraction. After processing, specimen information will be entered into Freezerworks specimen management software and barcode labels will be generated and placed on the specimens. As the specimens are placed in frozen storage at -80 degrees C, the tubes will be barcode wanded and a specimen map will be created for inventory and retrieval purposes. To ensure the safety of these specimens, CALM will use a monitored freezer on an emergency electrical power circuit with carbon dioxide back-up. Likewise, DNA will be extracted, aliquotted, and prepared for storage in a similar manner. CALM will perform hs-CRP, IL6, and TNF testing on retrieved aliquots. All testing will be performed in duplicate and with appropriate Quality Control procedures using commercial controls and duplicate specimens; the laboratory will also subscribe to available proficiency programs. CALM staff will oversee the delivery of specimens to the hospital laboratory for CK, Liver Profile and other tests as required; CALM staff will also retrieve the results of these tests and enter them into a computerized database for transfer to the data management staff.