Laboratory Rat Core

实验室大鼠核心

• 批准号: 6850622
• 负责人: PAUL M PLOTSKY
• 金额: $ 15.3万
• 依托单位: EMORY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6850622
• 关键词: animal care; behavioral /social science research tag; biomedical facility; clinical depression; disease /disorder model; early experience; gender difference; hypothalamic pituitary adrenal axis; laboratory rat; mother deprivation

The Rat Model Core (Core 9001) of the Emory University Silvio O. Conte Center for the Neuroscience of Mental Disorders (CCNMD) represents an integral component of the overall Center. This Core will serve as the source of all rodents used by individual preclinical Research Projects 1-4. This Core will produce and characterize variants of a rat early life stress (ELS) model associated with vulnerability to the development of an anxiety-and depression-like syndrome. Personnel will purchase or breed rodents, provide high quality animal care and uniformly applied neonatal manipulation protocols necessary to generate the ELS model, characterize the phenotype of all animals, ascertain the stage of the estrus cycle in adult female animals, and provide experimental manipulations as required by individual preclinical Research Projects within the Center. This ELS model consists of Long Evans rats which are exposed to different rearing conditions from postnatal days (PND) 2-12, including: (1) animal facility reared (AFR) colony controls, (2) brief handling plus 15 min maternal separation (HMS15) handling controls, (3) brief handling plus variable maternal separation (HMS180+). Comparison of data obtained from these standardized rearing conditions among the various basic Research projects of this CCNMD will permit identification of central neurocircuits and intracellular mechanisms which are impacted by early life stress and which give rise to this anxiety- and depression-like syndrome; furthermore, because the animal models will be standardized, regression modeling can be used to elucidate interactions among these CNS systems in the genesis of this condition. In addition, the efficacy of various manipulations or treatments on aspects of the anxiety- and depression-like syndrome will be tested in these models. An important aspect of these animals is comparison with the phenotype and other measures in the nonhuman primate model as well as in the human studies. To this end, Core 9001 will characterize the behavioral and HPA axis stress responsiveness of animals, provide phenotypic screening for anhedonia, anxiety-/fear-like behavior, as well as providing surgical services, resident intruder social defeat exposure, and other protocols as required by the Research Projects. This organization has the advantage of maintaining consistency in animal handling and implementation of all protocols so that each preclinical Research Project receives identically characterized and treated animals for study.

埃默里大学西尔维奥·孔特精神疾病神经科学中心(CCNMD)的大鼠模型核心(核心9001)是整个中心不可或缺的组成部分。该核心将作为个人临床前研究项目1-4使用的所有啮齿动物的来源。这一核心将产生并表征与易患焦虑和抑郁样综合症相关的大鼠早期生活应激(ELS)模型的变体。人员将购买或饲养啮齿动物，提供高质量的动物护理，并统一应用生成ELS模型所需的新生儿操作方案， 确定所有动物的表型特征，确定成年雌性动物的发情周期阶段，并根据中心内个别临床前研究项目的要求提供实验操作。该ELS模型由Long Evans大鼠组成，从出生后2-12天开始暴露于不同的饲养条件，包括：(1)动物设施饲养(AFR)群体对照，(2)短暂处理+15min母体分离(HMS15)处理对照，(3)短暂处理+可变母体分离(HMS180+)。将这些标准化养育条件在CCNMD的不同基础研究项目中获得的数据进行比较，将有助于识别受早期生活应激影响的中枢神经回路和细胞内机制，以及导致焦虑和抑郁样症状的因素 此外，由于动物模型将是标准化的，回归模型可以用来阐明在这种情况的发生中这些中枢神经系统之间的相互作用。此外，将在这些模型中测试各种手法或治疗方法对焦虑和抑郁样综合症的有效性。这些动物的一个重要方面是与非人灵长类动物模型以及人类研究中的表型和其他指标进行比较。为此，Core 9001将表征动物的行为和HPA轴应激反应，提供快感缺失、焦虑/恐惧样行为的表型筛选，以及提供外科服务、居民入侵者社会 失败暴露，以及研究项目所要求的其他方案。该组织的优势是在动物处理和所有方案的实施上保持一致性，以便每个临床前研究项目接收相同的特征和治疗的动物进行研究。