CORE--HISTOLOGY CORE

核心--组织学核心

• 批准号: 6828189
• 负责人: JAMES E FABER
• 金额: $ 11.23万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6828189
• 关键词: aging; biomedical facility; collagen; elastin; histology; immunocytochemistry; laboratory mouse; morphometry

The major function of this core will be to prepare materials that are necessary for histological and morphometric analysis of vascular tissue from the various types of mouse models that are to be analyzed in the three projects. The major functions of the core will include preparation of sections for routine H and E staining as well as Von Geisen staining, detailed morphometric analysis, calculations of lesion area and archiving of data. The core will also be responsible for preparing tissue for immtmohistochemical staining and for the performance of immunohistochemical staining of proteins that are likely to change during vascular aging such as type I and HI collagen and elastin. The core will conduct biochemical analyses of changes in these forms of collagen and elastin. They will analyze changes in distribution of different cell types within the vascular wall such as changes in inflammatory cells that are likely to be altered in certain models that are to be analyzed. Since all of these analyses will be performed in a centralized facility using standardized methodologies it should be possible to make cross comparisons particularly among morphologic and morphometric changes that occur in the various mouse models that have been prepared. Performance of these procedures in a centralized facility is also likely to greatly facilitate the rate of progress toward completion of the specifications in these projects and will provide an objective and unbiased interpretation of the results that are obtained.

该核心的主要功能是为三个项目中要分析的各种小鼠模型的血管组织的组织学和形态计量学分析准备必要的材料。核心的主要功能包括常规H、E染色和Von Geisen染色的切片准备，详细的形态计量学分析，病变面积计算和数据存档。该核心还将负责准备组织进行免疫组织化学染色，并对在血管老化过程中可能发生变化的蛋白质（如I型和HI型胶原蛋白和弹性蛋白）进行免疫组织化学染色。核心将对这些形式的胶原蛋白和弹性蛋白的变化进行生化分析。他们将分析不同细胞分布的变化