CORE--HISTOLOGY CORE

这个核心的主要功能将是准备对三个项目中要分析的各种类型的小鼠模型的血管组织进行组织学和形态计量学分析所需的材料。核心的主要功能将包括准备常规H和E染色的切片以及冯·盖森染色、详细的形态计量分析、病变面积的计算和数据存档。该核心还将负责准备组织进行免疫组织化学染色，并对可能在血管老化过程中发生变化的蛋白进行免疫组织化学染色，如I型和HI型胶原和弹性蛋白。核心将对这些形式的胶原和弹性蛋白的变化进行生化分析。他们将分析不同细胞分布的变化 血管壁内的类型，如炎性细胞的变化，在某些模型中可能会改变，这些模型将被分析。由于所有这些分析都将在一个使用标准化方法的中央设施中进行，应该可以进行交叉比较，特别是在已经准备好的各种小鼠模型中发生的形态和形态计量学变化之间。在中央设施中执行这些程序也可能极大地促进完成这些项目中的规范的进度，并将提供对 所获得的结果。

The major function of this core will be to prepare materials that are necessary for histological and morphometric analysis of vascular tissue from the various types of mouse models that are to be analyzed in the three projects. The major functions of the core will include preparation of sections for routine H and E staining as well as Von Geisen staining, detailed morphometric analysis, calculations of lesion area and archiving of data. The core will also be responsible for preparing tissue for immtmohistochemical staining and for the performance of immunohistochemical staining of proteins that are likely to change during vascular aging such as type I and HI collagen and elastin. The core will conduct biochemical analyses of changes in these forms of collagen and elastin. They will analyze changes in distribution of different cell types within the vascular wall such as changes in inflammatory cells that are likely to be altered in certain models that are to be analyzed. Since all of these analyses will be performed in a centralized facility using standardized methodologies it should be possible to make cross comparisons particularly among morphologic and morphometric changes that occur in the various mouse models that have been prepared. Performance of these procedures in a centralized facility is also likely to greatly facilitate the rate of progress toward completion of the specifications in these projects and will provide an objective and unbiased interpretation of the results that are obtained.