CORE--HISTOLOGY CORE

该核心的主要功能是制备三个项目中待分析的各种小鼠模型血管组织的组织学和形态学分析所需的材料。核心的主要功能将包括制备常规H和E染色以及Von Geisen染色的切片、详细的形态测定分析、计算病变面积和数据存档。该核心还将负责制备用于免疫组织化学染色的组织，并负责对血管老化期间可能发生变化的蛋白质（如I型和HI胶原蛋白和弹性蛋白）进行免疫组织化学染色。核心将进行生化分析的变化，这些形式的胶原蛋白和弹性蛋白。他们将分析不同细胞分布的变化， 血管壁内的类型，例如在待分析的某些模型中可能改变的炎性细胞的变化。由于所有这些分析都将在一个集中的设施中使用标准化方法进行，因此应该可以进行交叉比较，特别是在已制备的各种小鼠模型中发生的形态学和形态测量学变化之间。在一个集中的设施中执行这些程序也可能极大地促进这些项目中完成规范的进度，并将提供一个客观和公正的解释， 所获得的结果。

The major function of this core will be to prepare materials that are necessary for histological and morphometric analysis of vascular tissue from the various types of mouse models that are to be analyzed in the three projects. The major functions of the core will include preparation of sections for routine H and E staining as well as Von Geisen staining, detailed morphometric analysis, calculations of lesion area and archiving of data. The core will also be responsible for preparing tissue for immtmohistochemical staining and for the performance of immunohistochemical staining of proteins that are likely to change during vascular aging such as type I and HI collagen and elastin. The core will conduct biochemical analyses of changes in these forms of collagen and elastin. They will analyze changes in distribution of different cell types within the vascular wall such as changes in inflammatory cells that are likely to be altered in certain models that are to be analyzed. Since all of these analyses will be performed in a centralized facility using standardized methodologies it should be possible to make cross comparisons particularly among morphologic and morphometric changes that occur in the various mouse models that have been prepared. Performance of these procedures in a centralized facility is also likely to greatly facilitate the rate of progress toward completion of the specifications in these projects and will provide an objective and unbiased interpretation of the results that are obtained.