Imaging neuromodulation during locomotor ontogeny

运动个体发育过程中的成像神经调节

• 批准号: 6893984
• 负责人: David McLean
• 金额: $ 4.99万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2003
• 资助国家: 美国
• 起止时间: 2003-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6893984
• 关键词: behavior; bioimaging /biomedical imaging; brain stem; cell cell interaction; confocal scanning microscopy; dopamine; growth /development; immunocytochemistry; innervation; interneurons; motor neurons; neural transmission; neuroimaging; norepinephrine; postdoctoral investigator; psychomotor function; serotonin; spinal cord; synapses; zebrafish

DESCRIPTION (provided by applicant): Neuromodulation is extremely important as it can govern the way neurons interact with one another. This is true from the simplest to the most complex neural processes, be it locomotion or emotion. Investigations into vertebrate neuromodulation to date have primarily used pharmacological tools, where drugs are bath applied and their cellular and synaptic consequences are monitored electrophysiologically. However, these studies provide no direct information about the activity patterns of the cells that contain these chemicals or what the real behavioral consequences of that activity may be. This research project plans to gain unprecedented access to neuromodulation in a vertebrate preparation using the elegant imaging and genetic tools available in the zebrafish, Danio rerio. The zebrafish is poised to become a powerful model system in all aspects of motor control and its development and this project intends to capitalize on its advantages by focusing on the role of descending modulatory centers. Thus, the aim of this proposal is three-fold: 1) to identify and catalogue the development of three potential neuromodulators, serotonin, dopamine and noradrenaline, 2) to visualize the activity of their respective neurons during elicited axial motor behaviors, and 3) to perturb the activity of these neurons in vivo and monitor the behavioral consequences.

描述（由申请人提供）：神经调节非常重要，因为它可以控制神经元相互作用的方式。从最简单到最复杂的神经过程，无论是运动还是情感，都是如此。迄今为止，对脊椎动物神经调节的研究主要使用药理学工具，其中药物被应用于浴，并且它们的细胞和突触后果被电生理学监测。然而，这些研究没有提供关于含有这些化学物质的细胞的活动模式或这种活动的真实的行为后果的直接信息。该研究项目计划使用斑马鱼Danio rerio中可用的优雅成像和遗传工具，在脊椎动物制剂中获得前所未有的神经调节。斑马鱼有望成为运动控制及其发展各个方面的强大模型系统，本项目旨在通过关注下行调节中心的作用来利用其优势。因此，该提议的目的是三重的：1）识别和分类三种潜在的神经调节剂，5-羟色胺，多巴胺和去甲肾上腺素的发展，2）可视化它们各自的神经元在引起的轴向运动行为期间的活动，和3）扰乱这些神经元在体内的活动并监测行为后果。