The role of benedict in Drosophila cell cycle regulation

本尼迪克特在果蝇细胞周期调控中的作用

• 批准号: 6953950
• 负责人: JASON Z MORRIS
• 金额: $ 21.77万
• 依托单位: FORDHAM UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6953950
• 关键词: Drosophilidae; arthropod genetics; cell cycle; cell growth regulation; cell morphology; developmental genetics; gene complementation; gene expression; gene interaction; gene mutation; genetic mapping; genetic regulation; genetic translation; in situ hybridization; larva; ovary; polymerase chain reaction

DESCRIPTION (provided by applicant): My laboratory's long-term goal is to improve our understanding of cell cycle regulation during Drosophila development. This proposal focuses on the molecular identification and functional characterization of benedict (bene), a gene identified in a clonal screen I carried out as a post-doctoral fellow. The three known loss-of-function mutations in bene are lethal and disrupt endoreplication in the larval fat body and salivary gland, bene germ line clones exhibit nurse cell chromosome condensation defects and disruption of nurse cell endoreplication. The morphology of oocyte chromosomes in these clones is characteristic of meiotic disruption defects, bene mutations are not cell lethal and do not cause obvious mitotic defects. The suite of bene defects suggests that bene may play a unique role in endoreplication and meiotic cell cycle regulation, and mutations that disrupt M phase regulation, S phase regulation, and translation regulation in oocytes all exhibit different subsets of the bene phenotypes. We have mapped bene to a 300 kb, sequenced region of the genome that includes 31 candidate genes. Specific Aim 1: To identify which of the 31 candidate genes in the bene region is disrupted in the mutant bene alleles and to determine the bene expression pattern\ Specific Aim 2: To analyze bene function in the larva and ovary by analysis of cell cycle markers and by assays for genetic interaction with cell cycle regulators.

描述（由申请人提供）：我实验室的长期目标是提高我们对果蝇发育过程中细胞周期调控的理解。这个建议集中在分子鉴定和功能特性的benedict（贝内），一个基因在克隆筛选中确定我作为博士后研究员进行。三个已知的贝内功能缺失突变是致命的，并破坏幼虫脂肪体和唾液腺中的内复制，贝内生殖系克隆表现出护士细胞染色体凝聚缺陷和护士细胞内复制的破坏。这些克隆的卵母细胞染色体形态具有减数分裂破坏缺陷的特征，贝内突变不是细胞致死性的，不引起明显的有丝分裂缺陷。这一系列贝内缺陷表明贝内可能在核内复制和减数分裂细胞周期调控中发挥独特作用，并且破坏卵母细胞中M期调控、S期调控和翻译调控的突变都表现出贝内表型的不同子集。我们已经将贝内定位到300 kb的基因组测序区域，其中包括31个候选基因。 具体目标1：为了鉴定贝内区31个候选基因中的哪一个在突变贝内等位基因中被破坏，并确定贝内表达模式， 具体目标二：通过分析细胞周期标志物和细胞周期调节因子的遗传相互作用来分析幼虫和卵巢的贝内功能。

项目成果

Mutations in the Drosophila mitochondrial tRNA amidotransferase, bene/gatA, cause growth defects in mitotic and endoreplicating tissues.

果蝇线粒体 tRNA 酰胺转移酶 bene/gatA 的突变会导致有丝分裂和内复制组织的生长缺陷。

• DOI: 10.1534/genetics.107.084376
• 发表时间: 2008
• 期刊: Genetics
• 影响因子: 3.3
• 作者: Morris,JasonZ;Bergman,Leah;Kruyer,Anna;Gertsberg,Mikhail;Guigova,Adriana;Arias,Ronald;Pogorzelska,Monika
• 通讯作者: Pogorzelska,Monika