IMPROVED HOUSING AND PRODUCTION OF TRANSGENIC MICE AT VU

维多利亚大学改善转基因小鼠的饲养和生产

• 批准号: 6756149
• 负责人: JOAN T. RICHERSON
• 金额: $ 63.05万
• 依托单位: VANDERBILT UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-04-01 至 2005-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6756149
• 关键词: animal colony; biomedical equipment purchase; cryopreservation; genetically modified animals; laboratory mouse; lyophilization

DESCRIPTION (provided by applicant): Funds provided by the NCRR Grant 1C06RR7400-01 have facilitated the build out of the seventh floor shell space in the MCN II Animal Facility. This new space will provide housing for an additional 10,000 cages of mice and for expansion of the Transgenic Mouse/ES Cell Shared Resource. This growth is essential for the continued success of the research enterprise at Vanderbilt University (VU). For three years, the mouse census has increased by 2000 cages annually; currently the actual mouse census is ahead of the July 2003 projections. To accommodate the expanding mouse population, additional ventilated cage and rack units must be purchased. Compared to a static system, ventilated cage and rack units offer the advantage of housing a larger number of mouse cages in the same floor space. This application requests funding to purchase 25 single sided and 12 double-sided ventilated cage and rack units. VU is committed to converting all housing of mice to ventilated cage and rack units. These ventilated cages will ease the overcrowding in other existing animal facilities, improve the microenvironment of the mice, and reduce personnel exposure to mouse allergens. The research programs of 57 PHS funded investigators will directly benefit from the availability of these ventilated units. Many investigators (including the 57 mentioned above) utilize germ-line altered mice in their research. The Transgenic Mouse and ES Cell Shared Resource has been instrumental in providing services to produce and maintain these unique mouse strains. Expansion of the services offered by this resource will be enhanced by the availability of additional equipment. Funding is requested to purchase two Vertical Flow Stations, two Taylor Wharton Liquid Nitrogen small tank dewars, two roller bases for dewars, two low level battery alarms, a Xenoworks Automatic Transjector with Manipulators, and a Labconco Freeze Dry System. This equipment is important to VU's continuing effort to provide new services such as Intracytoplasmic Sperm Injection (ICSI) and maintain the cryopreservation of sperm and embryos.

描述（由申请人提供）： 由NCRR赠款1C 06 RR 7400 -01提供的资金促进了MCN II动物设施七楼外壳空间的建造。这个新的空间将提供额外的10，000笼小鼠的住房，并扩大转基因小鼠/ES细胞共享资源。这种增长是必不可少的研究企业在范德比尔特大学（VU）的持续成功。三年来，老鼠普查每年增加2 000个笼子;目前实际的老鼠普查比2003年7月的预测要早。为了适应不断扩大的小鼠种群，必须购买额外的通风笼和机架单位。与静态系统相比，通风笼和机架单元的优点是在相同的地板空间中容纳更多数量的鼠笼。该申请要求提供资金，以购买25个单面和12个双面通风笼和机架单元。似曾相识是致力于转换所有住房的小鼠通风笼和机架单位。这些通风笼将缓解其他现有动物设施的过度拥挤，改善小鼠的微环境，并减少人员暴露于小鼠过敏原。57个PHS资助的研究人员的研究计划将直接受益于这些通风装置的可用性。 许多研究者（包括上面提到的57人）在他们的研究中使用生殖系改变的小鼠。转基因小鼠和ES细胞共享资源在提供服务以生产和维持这些独特的小鼠品系方面发挥了重要作用。增加设备将有助于扩大这一资源提供的服务。申请的资金用于购买两个垂直流动站、两个Taylor Wharton小型液氮罐杜瓦瓶、两个杜瓦瓶辊座、两个低液位电池报警器、一个带操纵器的Xenoworks自动发射器和一个Labconco冷冻干燥系统。该设备是重要的似曾相识的持续努力，提供新的服务，如卵胞浆内单精子注射（ICSI）和维持精子和胚胎的冷冻保存。