CORE--MAGNETIC RESONANCE

请求为磁共振核心提供支持。项目2和3中提出的实验主要针对产生与膜界面和脂类相互作用的蛋白质结构域的中分辨率和高分辨率结构数据。该核心将为设计和实施共振实验(基于EPR和核磁共振)提供设备和专业知识，以生成这些结构。将采用在界面确定蛋白质结构所独有的一些专门方法，对于这些方法，PI和高级科学家(Jeffrey F.Ellena博士)和顾问都有相当的专业知识。杰夫·埃莱纳博士目前在弗吉尼亚大学化学系管理磁共振设备。他的部分时间将用于培训学生和科学家如何使用EPR设备和分析EPR数据。他还将准备膜模拟样品，并进行核磁共振实验，以确定蛋白质结构域在脂类界面中的定位。Ellena博士将与PI以及John Bushweller教授就实验的设计和解释进行互动。Bushweller博士将在样品的准备、标记多肽的表达以及高分辨率线条缩小核磁共振方法的实施方面担任顾问。

Support is requested for a magnetic resonance core. Experiments that are proposed mainly in projects 2 and 3 are directed at generating medium and high-resolution structural data of protein domains interacting with membrane interfaces and lipids. This core will provide both equipment and expertise for the design and implementation of resonance experiments (both EPR and NMR based) to generate these structures. A number of specialized methods, unique to the determination of protein structures at interfaces, will be employed for which the PI and senior scientist (Dr. Jeffrey F. Ellena) and consultant have considerable expertise. Dr. Jeff Ellena currently manages the magnetic resonance facility in the Chemistry Department at UVa. A portion of his time will be made available to train students and scientists in the program in the use of EPR equipment and in the analysis of EPR data. He will also be available to prepare membrane mimetic samples and execute NMR experiments directed at determining the localization of protein domains within lipid interfaces. Dr. Ellena will interact both with the PI, and with Prof. John Bushweller on the design and interpretation of experiments. Dr. Bushweller will act as a consultant for assistance in the preparation of samples, the expression of labeled peptides, and in the implementation of high-resolution line-narrowing NMR methods.