CORE--MAGNETIC RESONANCE

要求支持一个磁共振磁芯。项目2和3中提出的实验主要针对生成与膜界面和脂质相互作用的蛋白质结构域的中分辨率和高分辨率结构数据。该核心将为共振实验（EPR和NMR为基础）的设计和实施提供设备和专业知识，以生成这些结构。将采用一些专门的方法，以确定界面处的蛋白质结构，PI和高级科学家（Jeffrey F. Ellena博士）和顾问具有相当的专业知识。Jeff Ellena博士目前管理着弗吉尼亚大学化学系的磁共振设备。他的一部分时间将用于培训该项目的学生和科学家使用EPR设备和分析EPR数据。他还将准备模拟膜样品，并执行核磁共振实验，以确定脂质界面内蛋白质结构域的定位。Ellena博士将与PI和John Bushweller教授就实验的设计和解释进行互动。Bushweller博士将担任顾问，协助准备样品，标记肽的表达，并在高分辨率线窄核磁共振方法的实施。

Support is requested for a magnetic resonance core. Experiments that are proposed mainly in projects 2 and 3 are directed at generating medium and high-resolution structural data of protein domains interacting with membrane interfaces and lipids. This core will provide both equipment and expertise for the design and implementation of resonance experiments (both EPR and NMR based) to generate these structures. A number of specialized methods, unique to the determination of protein structures at interfaces, will be employed for which the PI and senior scientist (Dr. Jeffrey F. Ellena) and consultant have considerable expertise. Dr. Jeff Ellena currently manages the magnetic resonance facility in the Chemistry Department at UVa. A portion of his time will be made available to train students and scientists in the program in the use of EPR equipment and in the analysis of EPR data. He will also be available to prepare membrane mimetic samples and execute NMR experiments directed at determining the localization of protein domains within lipid interfaces. Dr. Ellena will interact both with the PI, and with Prof. John Bushweller on the design and interpretation of experiments. Dr. Bushweller will act as a consultant for assistance in the preparation of samples, the expression of labeled peptides, and in the implementation of high-resolution line-narrowing NMR methods.