Development of scalable animal-free bioprocesses for cultivated meat production

开发用于培育肉类生产的可扩展的无动物生物工艺

• 批准号: 2733838
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2022
• 资助国家: 英国
• 起止时间: 2022 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2733838
• 关键词: Development; scalable; animal; free; bioprocesses

There is an increased need for sustainable, protein rich food sources to support the rapidly growing population. The current agricultural practices to produce meat are unethical and unsustainable as they utilise a high proportion of natural resources (land, water) and moreover are responsible for ~14.5% of the total greenhouse gas emissions. Alternative protein technologies such as cultivated meat can provide a sustainable solution. Cultivated meat is animal meat that is produced in a bioreactor under controlled conditions from animal cells obtained through a biopsy. Many prototype cultivated meat products are currently grown using materials derived from animals such as foetal bovine serum which requires animal slaughter and is not sustainable. To maximise the positive environmental and animal welfare outcomes of cultivated meat, all animal-derived components should be replaced by sustainable alternatives. This project aims to develop a sustainable, scalable and animal-free manufacturing process for cultivated meat production. This will be achieved by:1) expansion and differentiation of porcine mesenchymal stem cells and porcine myoblasts in monolayer;2) developing scalable processes for expansion and differentiation of these cells in bioreactors; 3) replacing animal-derived components by testing conventional growth factor cocktails, as well as sustainable materials inspired from a) foods, dietary practices and cultures such as plant-based hydrolysates, high protein content flours and powders (e.g meal worm flour, yeast extract, nutritional yeast - vegan replacements, snow fungus powder) and b) fitness practices such as high content protein powders, aminoacids, creatine etc. This project will result in the development of animal-free media formulations and scalable processes for manufacturing porcine stem cells for cultivated meat. These findings will be beneficial to the entire cultivated meat industry as it will provide solutions to the current challenges that are hindering the commercialisation of these types of products. This project aligns with EPSRC's research areas of manufacturing technologies and process systems.

人们越来越需要可持续的、富含蛋白质的食物来源来支持快速增长的人口。目前生产肉类的农业做法是不道德和不可持续的，因为它们占用了很大比例的自然资源（土地，水），而且还占温室气体排放总量的14.5%。替代蛋白质技术，如人造肉，可以提供可持续的解决方案。培养肉是在生物反应器中在受控条件下从通过活组织检查获得的动物细胞生产的动物肉。目前，许多原型养殖肉制品都是使用来自动物的材料（如胎牛血清）种植的，这需要屠宰动物，并且是不可持续的。为了最大限度地发挥养殖肉类对环境和动物福利的积极影响，所有动物源性成分都应被可持续替代品取代。该项目旨在开发一种可持续的，可扩展的和无动物的养殖肉类生产工艺。这将通过以下方式实现：1）在单层中扩增和分化猪间充质干细胞和猪成肌细胞;2）开发用于在生物反应器中扩增和分化这些细胞的可扩展方法; 3）通过测试常规的生长因子混合物来替代动物源性组分，以及从a）食品、饮食实践和文化（如植物基水解产物）中获得的可持续材料，高蛋白面粉和粉末（例如粗粉虫粉、酵母提取物、营养酵母-素食替代品、雪菌粉）和B）健身实践如高含量蛋白粉，氨基酸，该项目将导致开发无动物培养基配方和可扩展的生产用于培养肉类的猪干细胞的工艺。这些发现将有利于整个养殖肉类行业，因为它将为当前阻碍这些类型产品商业化的挑战提供解决方案。该项目与EPSRC的制造技术和工艺系统的研究领域保持一致。