Controlled Release Bone Morphogenic Protein/Nano-calcium

我们提出了一种新的方法，将基于纳米相磷酸钙和骨形态发生蛋白（BMP）混合物的载药涂层沉积在金属植入物上。这种涂层的一个独特之处在于，它会在手术后以一种可控的方式释放药物。此外，涂层由纳米级磷酸钙特征组成，我们已经在初步研究中表明，它可以显著增强成骨细胞的功能。在第一阶段的可行性研究中，三种纳米磷酸钙涂层配方（非晶、半晶和结晶）将在普渡大学合成。然后，纳米相磷酸钙将与BMP混合，并使用我们的新沉积工艺涂覆在钛基上。这些样品上的涂层将被评估释放速率，目的是复制我们之前从纳米capcvbmp材料的颗粒形式成功获得的结果。在控制释放速率被证明后，我们将使用成骨细胞反应性试验来评估蛋白质在体外的生物活性，这种方法在纳米磷酸钙材料上有和没有BMP。采用SEM、XRD、AFM和EDS等分析方法研究涂层样品的各种结构和材料特性。在第二阶段，将在体内动物模型中评估植入物上的纳米capcvbmp涂层。

DESCRIPTION (provided by applicant): Controlled Release of Bone Morphogenic Protein using Nano-Calcium Phosphate Coatings TP-2004-71 We propose a novel method for depositing a drug-loaded coating based on a mixture of nano-phase calcium phosphate and bone morphogenic protein (BMP) on metal implants. One unique feature of the coating is that it will release the drug in a controlled fashion following the surgical procedure. Additionally, the coating is made up of nano-sized calcium phosphate features, which we have shown in preliminary studies to significantly enhance osteoblast function. In Phase I feasibility studies, three nano-calcium phosphate coating formulations (amorphous, semi- crystalline and crystalline) will be synthesized at Purdue University. Nano-phase calcium phosphate will then be mixed with BMP and coated onto titanium substrates using our novel deposition process. The coating on these samples will be evaluated for rate of release with the goal of reproducing our successful previous results from pelletized form of nano-CaPCVBMP material. After controlled rate of release has been demonstrated, we will assess protein bioactivity in vitro using osteoblast reactivity assay on nano-calcium phosphate material with and without BMP. Various structural and material characteristics of the coated samples will be studied using SEM, XRD, AFM and EDS. In Phase II, nano-CaPCVBMP coatings on implants will be evaluated in an in vivo animal model.