Core--Cryopreservation and Embryo Derivation

核心--冷冻保存与胚胎衍生

• 批准号: 7205333
• 负责人: BRUCE A BEUTLER
• 金额: $ 28.07万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2011-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7205333
• 关键词: artificial fertilization; biomedical facility; cryopreservation; embryo /fetus preservation; gene mutation; genetic mapping; genetic strain; genetic susceptibility; genetically modified animals; host organism interaction; immunogenetics; in vitro fertilization; laboratory mouse; microorganism immunology; virus diseases

This Core is intended to: 1. permit rapid and safe exchange of germline mutant mice between La Jolla and Osaka as frozen embryos, circumventing the delay imposed by quarantine; 2. secure the valuable strains created using ENU; 3. provide for the rescue of strains with low fertility and accelerate the fixation of mutations that can only be fixed by inbreeding, given that the MCMV screen is lethal to homozygotes; 4. provide for the distribution of mutant strains to the scientific community at large by expanding and shipping homozygous stocks to the MMRRC. This Core will be run using techniques that we have established and have used routinely, and requires no expenditure for equipment. It will be mirrored by a comparable Core in Osaka, Japan, which will focus primarily upon items 1, 2, and 4 (above) to the exclusion of IVF. Neither Core can function in the absence of the other, since embryos frozen in one Core must be re-derived in the other, and vice versa. Hence they should each be viewed as separate but essential Cores for two of the participating groups (in La Jolla and Osaka).

该核心旨在：1。允许在拉霍亚和 大坂作为冷冻胚胎，规避检疫造成的延误; 2.确保有价值的菌株 使用ENU创建; 3.提供低生育力菌株的拯救，并加速固定 鉴于MCMV筛选对纯合子是致命的，只能通过近亲繁殖来固定的突变; 4. 通过扩大和运输向广大科学界分发突变菌株 纯合子原种到MMRRC。这个核心将使用我们已经建立的技术运行， 常规使用，不需要设备支出。它将被一个类似的核心镜像， 日本大坂，主要关注项目1、2和4（上文），不包括IVF。既不 核心可以在没有另一个核心的情况下发挥作用，因为冷冻在一个核心中的胚胎必须在另一个核心中重新衍生。 另一个，反之亦然。因此，它们应该被视为两个独立但必不可少的核心， 参加团体（在拉霍亚和大坂）。