Refining Affinity-Based Mass Photometry

蛋白质作为控制几乎所有生命过程的复杂网络的一部分相互作用。为了理解这些相互作用网络，我们必须首先确定单个蛋白质的相互作用，然后再量化它们。重要的是，我们希望在尽可能与其原生环境相似的条件下研究这些相互作用。虽然存在用于此目的的几种方法，但它们具有限制，这阻碍了它们准确定量广泛的蛋白质相互作用的能力。这项研究将开发和完善基于亲和力的质量光度法的实验和分析工作流程：一种新型的基于单分子的方法，可以直接从裂解细胞和均质化组织中检测和测量特定蛋白质的质量。将基于亲和力的质量光度法与基于质谱的蛋白质组学相结合，将使我们能够在密切模拟细胞或组织环境的条件下检测、鉴定和定量特定的蛋白质相互作用。这将有助于更好地了解生物过程。BBSRC的主要优先领域：生物科学技术开发

Proteins interact as part of a complex network that controls almost all processes of life. In order to understand these interaction networks, we must first identify individual protein interactions, before quantifying them. Importantly, we want to study these interactions under conditions that are as similar to their native environment as possible. Whilst several methods exist for this purpose, they have limitations that hinder their ability to accurately quantify a wide range of protein interactions. This research will develop and refine experimental and analytical workflows for affinity-based mass photometry: a novel, single molecule-based method that can detect and measure the mass of specific proteins of interest, directly from lysed cells and homogenised tissues. Combining affinity-based mass photometry with mass spectrometry-based proteomics will enable us to detect, characterise and quantify specific protein interactions under conditions that closely mimic the cellular or tissue environment. This will lead to a better understanding of biological processes. Key BBSRC Priority Area: Technology development for the biosciences