CORE--TRANSGENIC MICE

核心——转基因小鼠

• 批准号: 7086848
• 负责人: Louis French Reichardt
• 金额: $ 18.82万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7086848
• 关键词: animal colony; biomedical facility; genetically modified animals; laboratory mouse; neural plasticity; synapses; synaptogenesis

The majority of projects in this program project grant will rely heavily on transgenic mice, some of which have already been generated and others of which need to be generated. During the past five years, existence of this core has greatly facilitated the ability of the laboratories directed by Rob Edwards, David Copenhagen, Louis Reichardt and Michael Stryker to exploit this powerful technology. During the next five years, we anticipate expanded use of this core that will almost certainly be used by all members of our new program project team. The existence of a core has made it possible to hire a senior specialist with more than 15 years of relevant experience in preparing transgenic mice plus a junior technician able to manage the mouse colonies and perform routine genotyping, etc. The former person has helped our laboratories generate more than a dozen transgenic lines and half a dozen knockout strains. The more junior technician has been particularly valuable in helping the labs entering this field. All laboratories will continue to benefit from a transgenic core with a person dedicated to performing well certain tasks of critical importance in generating such animals. To help culture and screen ES cells, provide transgenic animals with targeted mutations and provide help in training postdoctoral fellows and graduate students in this important technology, we propose to maintain a modestly-sized and staffed core facility with a cell culture facility for embryonic stem cells, equipment for injection of single cell embryos and blastocysts, and a mouse colony with fertile and vasectomized males and fertile females for use as single cell embryo or blastocyst donors and pseudopregnant recipients. The Reichardt laboratory will provide the supervision and expertise for setting up and managing this facility under the overall guidance of the Principal Investigator.

该项目的大多数项目将严重依赖转基因小鼠，其中一些已经产生，另一些需要产生。在过去的五年中，这个核心的存在极大地促进了由罗布·爱德华兹、大卫·哥本哈根、路易斯·赖克哈特和迈克尔·斯特赖克领导的实验室利用这一强大技术的能力。在接下来的五年里，我们预计这个核心的使用范围将扩大，几乎可以肯定，我们新项目团队的所有成员都将使用这个核心。核心的存在使得我们能够聘请一位在制备转基因小鼠方面具有15年以上相关经验的高级专家，以及一位能够管理小鼠集落和进行常规基因分型等的初级技术人员。越是初级技术人员 在帮助实验室进入这一领域方面特别有价值。所有实验室将继续受益于一个转基因核心，该核心有一个人致力于很好地执行某些在生产此类动物方面至关重要的任务。为了帮助培养和筛选ES细胞，提供具有靶向突变的转基因动物，并在这一重要技术方面为博士后研究员和研究生的培训提供帮助，我们建议维持一个中等规模和人员配备的核心设施，包括胚胎干细胞细胞培养设施，单细胞胚胎和囊胚注射设备，以及具有可育的和输精管切除的雄性和可育的雌性的小鼠群体，用作单细胞胚胎或胚泡供体和假孕受体。Reichardt实验室将在主要研究者的总体指导下，为建立和管理该机构提供监督和专业知识。