Molecular Imaging of cancer cells

癌细胞的分子成像

• 批准号: 7057801
• 负责人: GUANGWEN WAYNE ZHOU
• 金额: $ 16.58万
• 依托单位: LOUISIANA STATE UNIV A&M COL BATON ROUGE
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-22 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7057801
• 关键词: adenocarcinoma; bioimaging /biomedical imaging; bioinformatics; biomarker; biotechnology; breast neoplasms; cancer registry /resource; colorectal neoplasms; computer system design /evaluation; confocal scanning microscopy; data management; human tissue; image processing; immunocytochemistry; microarray technology; molecular /cellular imaging; molecular biology information system; neoplasm /cancer classification /staging; neoplasm /cancer diagnosis; neoplastic cell; peptide library; prostate neoplasms; protein localization; proteomics; technology /technique development

DESCRIPTION (provided by applicant): This proposal is a response to the PA-04-095, "novel technologies for in vivo imaging". We want to develop an antibody microarray staining technology to simultaneously obtain the molecular images of hundreds even thousands of proteins in different cancer cells and tissue samples. The novel antibody microarray (called dissociable antibody microarray) is different from the conventional capture microarray, as the antibodies were delivered to specific locations of targeted samples. In collaboration with Hypromatrix Inc., we have developed a prototype type of this protein microarray-"dissociable antibody microarray" and have applied the "dissociable antibody microarray" in antibody microarray staining technology, which has combined the power of immunohistochemical staining and the parallel analysis of antibody microarrays. This innovative technology can provide a novel approach in global analysis of protein expressions and protein subcellular localizations. In this proposal, we shall develop and expand this technique, so that it can be utilized to determine the molecular images of 400-1000 proteins among different cancer cells and tissues. We also propose to setup a prototype image database for molecular images of 400-1000 proteins in cell line and tissue samples and develop a database management system that can conveniently retrieve those images and compare them under different conditions will be setup and evaluated. We shall also use three kinds of adenocarcinoma originated cancers (breast cancer, colorectal cancer and prostate cancer) as a model system to identify novel molecular biomarkers for cancer diagnosis and for clinical stage assignment, by using the antibody microarray staining technique.

描述（由申请人提供）：

项目成果

Protein subcellular localization profiling of breast cancer cells by dissociable antibody microarray staining.

• DOI: 10.1002/pmic.200900585
• 发表时间: 2010-04
• 期刊: PROTEOMICS
• 影响因子: 3.4
• 作者: Fu, Guanyuan;Song, X. Cynthia;Yang, Xufen;Peng, Tao;Wang, Yingjian;Zhou, G. Wayne
• 通讯作者: Zhou, G. Wayne