The Role of Glutamate and CCK in the PBN in Taste Reactivity

PBN 中谷氨酸和 CCK 在味觉反应中的作用

• 批准号: 7116851
• 负责人: Michael Stephen King
• 金额: $ 3.32万
• 依托单位: STETSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-01 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7116851
• 关键词: behavior test; behavioral /social science research tag; cholecystokinin; glutamate receptor; laboratory rat; neural information processing; neurons; nutrient intake activity; nutrition related tag; parabrachial nucleus; psychomotor function; psychomotor reaction time; psychophysiology; stimulus /response; taste; videotape /videodisc

DESCRIPTION (provided by applicant): The long-term goal of this research is to elucidate the central neural mechanisms underlying feeding-related behaviors initiated by taste input in the rat. Recent studies have focused on the neurochemistry of two brainstem areas that receive gustatory sensory information, the rostral nucleus of the solitary tract and the parabrachial nucleus (PBN). The role of the PBN in the processing of sensory information and the initiation of oromotor responses is particularly intriguing since neurons in the PBN process taste-related sensory information and project to the network of motor neurons in the medulla that controls oromotor behaviors. Suggesting a role for glutamatergic neurotransmission within the PBN in the initiation of oromotor behaviors, microinjection of glutamate directly into the taste regions of the PBN in conscious rats elicits ingestive behaviors like mouth movements and tongue protrusions. Therefore, the first specific aim of the proposed study is to determine the role of glutamate receptors within the PBN in oromotor behaviors elicited by taste stimulation. It is hypothesized that the activitation of glutamate receptors is necessary for normal behavioral responses. Cholecystokinin (CCK) is a gut peptide that has satiety effects. There is some evidence that CCK modulates ingestive behaviors but a specific functional role for CCK and its receptors within the PBN has not been addressed. Therefore, the second specific aim of the proposed study is to determine the role of CCK in the PBN in oromotor behaviors elicited by intra-oral infusion of taste solutions in conscious rats. The hypothesis is that, consistent with its role in satiety, CCK will reduce ingestive oromotor responses to taste input. Both specific aims will be addressed by implanting cannula into the PBN as well as into the oral cavity so that glutamate or CCK receptor blockers can be injected into the PBN immediately prior to infusing taste solutions into the oral cavity in conscious rats. The proposed research is relevant to public health because it will improve the understanding of the neural circuits within the brainstem that control behavioral responses to taste input. These behavioral responses are critical to survival since they result in the acceptance of appropriate foods and rejection of potential toxins. Specifically, data collected during the proposed investigation will determine if glutamate and CCK in the PBN play a critical role in the assessment of ingested substances and subsequent behavioral responses.

描述（由申请人提供）：这项研究的长期目标是阐明大鼠味道输入引发的与喂养相关行为的基础神经机制。最近的研究集中在接收味觉信息的两个脑干区域的神经化学上，即单生间核的主核和副核核（PBN）。 PBN在感觉信息处理和Oromotor反应的启动中的作用尤其引人入胜，因为神经元在PBN过程中与味觉相关的感觉信息以及控制延髓中运动神经元网络的项目控制了Oromotor行为。提出了PBN中谷氨酸能神经传递在启动Oromotor行为中的作用，在有意识的大鼠中直接将谷氨酸直接注射到PBN的味道区域中，引发了摄入的行为，例如喉咙运动和舌头。因此，拟议的研究的第一个具体目的是确定谷氨酸受体在PBN中在味觉刺激引起的Oromotor行为中的作用。假设谷氨酸受体的激活对于正常行为反应是必要的。胆囊菌（CCK）是一种具有饱腹感的肠道肽。有证据表明，CCK调节了摄入行为，但是尚未解决CCK及其受体的特定功能作用。因此，拟议的研究的第二个具体目的是确定CCK在PBN中的作用在有意识大鼠中口腔内输注味道溶液引起的Oromotor行为中的作用。假设是，与其在饱腹感中的作用一致，CCK将减少对口味输入的摄入剂反应。通过将套管植入PBN以及口腔腔，以解决两个具体目标，以便可以在有意识大鼠的口腔中注入味觉溶液，然后将谷氨酸或CCK受体阻滞剂直接注入PBN。拟议的研究与公共卫生有关，因为它将提高对控制对味觉投入的行为反应的脑干内神经回路的理解。这些行为反应对于生存至关重要，因为它们导致接受适当的食物和拒绝潜在毒素。具体而言，在拟议的研究期间收集的数据将确定PBN中的谷氨酸和CCK在评估摄入物质和随后的行为反应中是否起关键作用。