Sensor for Paralytic Shellfish Poisons

麻痹性贝类毒物传感器

• 批准号: 7157046
• 负责人: ROBERT E. GAWLEY
• 金额: $ 10万
• 依托单位: WHITE RIVER BIOSCIENCE, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-08 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7157046
• 关键词: algae; animal extract; chemical synthesis; covalent bond; fish poison; fluorescence; glass; marine toxins; paralysis; saxitoxin; seafood poisoning; surface coating; surface property; technology /technique development; water sampling /testing

DESCRIPTION (provided by applicant): Harmful Algal Blooms, or "HAB's", are linked to many cases of human poisoning each year. Economic losses and costs to the fishing industry, public health, and tourism are estimated to be $40,000,000 annually, as of the late 1990s. 1 such HAB causes paralytic shellfish poisoning (PSP). Following an algal bloom, the toxins released by the microbes may accumulate in filter-feeding shellfish, consumption of which then causes disease symptoms, the most severe of which is respiratory paralysis and death. PSPs are 1 of only a few marine toxins that can be fatal to humans when consumed in food. This proposal seeks funding to develop recently reported technology for the fluorescence detection of PSPs into fluorescent detector plates. In Phase I, the limits of detection of 4 PSPs will be determined, and a preliminary evaluation of fluorescence response in toxic shellfish extracts will be conducted. Such plates will be adapted for use in a portable device in Phase II. The Specific Aims of this project are: 1. Synthesize fluorescent chemosensors suitable for covalent attachment to a glass slide. 2. Self-assembly and covalent attachment of the sensors to glass slides and evaluation of the surface properties and coating. 3. Evaluation of the fluorescence amplification to solutions of saxitoxin, PSP toxins, and toxic shellfish extracts. Paralytic Shellfish Poisons (PSPs) enter the human food chain most commonly through filter-feeding shellfish transvectors. The most toxic PSP, saxitoxin, is also 1 of only 3 "small molecules" on the government's list of "Select Agents". Fast, inexpensive means of detection PSPs is desirable.

描述（由申请人提供）：有害藻华，或“HAB”，每年与许多人类中毒病例有关。截至20世纪90年代末，渔业、公共卫生和旅游业的经济损失和成本估计为每年4 000万美元。1这类赤潮会导致麻痹性贝类中毒。藻类大量繁殖后，微生物释放的毒素可能积聚在滤食性贝类中，食用后会引起疾病症状，其中最严重的是呼吸麻痹和死亡。PSP是少数几种在食物中食用时可能对人类致命的海洋毒素之一。该提案寻求资金，以开发最近报道的将PSP荧光检测到荧光检测板中的技术。在第一阶段，将确定4种PSP的检测限，并对有毒贝类提取物的荧光响应进行初步评价。在第II阶段，此类板将适用于便携式装置。该项目的具体目标是：1。合成适合于共价连接到载玻片上的荧光化学传感器。2.传感器与载玻片的自组装和共价连接以及表面性质和涂层的评价。3.对石房蛤毒素、PSP毒素和有毒贝类提取物溶液的荧光放大评价。麻痹性贝类毒素（PSP）进入人类食物链最常见的是通过滤食性贝类transvector。最有毒的PSP，石房蛤毒素，也是政府“选择代理人”名单上仅有的3种“小分子”之一。需要快速、廉价的PSP检测手段。