Developing and applying fluorescent nanosensors for biological measurement.

荧光纳米传感器是惰性的、多功能的生物传感器，可用于在生命物理界面的微环境中对关键分子和离子进行重要测量，是动态过程实时测量的理想选择。在诺丁汉大学，荧光纳米传感器已经被开发出来，用于量化复杂模型系统中的pH、[2]、分子氧[3]和温度[4]。在这些传感器中，ph敏感的荧光纳米传感器获得了最大的发展势头。它们已经在一系列复杂和多样的微环境中进行了评估和验证，并通过绘制线虫模式生物（秀丽隐杆线虫[5]和太平洋Pristionchus[6]）的酸化图，阐明酿酒酵母[7]的亚细胞发酵途径，测定了人间充质干细胞（hMSCs）中外来物质的细胞内加工[8]，并表征了细菌生物膜生长过程中酸副产物的进化[9]。该项目将继续将ph敏感荧光纳米传感器应用于多种生物环境，包括果蝇（Drosophila melanogaster）、泰利芥（拟南芥）和CHO/Heck 293细胞培养进行生物处理。以及开发新的生物传感器的关键生物分子和离子。

Fluorescent nanosensors are inert, versatile biosensors, that can be used to make important measurements of key molecules and ions in microenvironments at the physics of life interface and are ideal for real-time measurements of dynamic processes [1].At the University of Nottingham, fluorescent nanosensors have been developed to quantify pH, [2] molecular oxygen [3] and temperature [4] in complex model systems. Of these sensors, the pH-sensitive fluorescent nanosensors have gathered the greatest momentum. They have been evaluated and validated in a range of complex and diverse microenvironments and demonstrated their immense potential by mapping the acidification in nematode model organisms (Caenorhabditis elegans [5] & Pristionchus pacificus [6]), elucidation of subcellular fermentation pathways in Saccharomyces cerevisiae [7], determined the intracellular processing of foreign material in human mesenchymal stem cells (hMSCs) [8] & characterised the evolution of acid by-products during bacterial biofilm growth [9].This project will continue the application of pH-sensitive fluorescent nanosensors to diverse biological environments, which include fruit flies (Drosophila melanogaster), Thale cress (Arabidopsis thaliana) and CHO/Heck 293 cell culture for bioprocessing. As well as develop new biosensors for key biological molecules and ions.