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Developing and applying fluorescent nanosensors for biological measurement.

开发和应用用于生物测量的荧光纳米传感器。

基本信息

批准号：
2739956
负责人：
金额：
--
依托单位：
University of Nottingham
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2022
资助国家：
英国
起止时间：
2022 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2739956
关键词：
Developing applying fluorescent nanosensors biological

项目摘要

Fluorescent nanosensors are inert, versatile biosensors, that can be used to make important measurements of key molecules and ions in microenvironments at the physics of life interface and are ideal for real-time measurements of dynamic processes [1].At the University of Nottingham, fluorescent nanosensors have been developed to quantify pH, [2] molecular oxygen [3] and temperature [4] in complex model systems. Of these sensors, the pH-sensitive fluorescent nanosensors have gathered the greatest momentum. They have been evaluated and validated in a range of complex and diverse microenvironments and demonstrated their immense potential by mapping the acidification in nematode model organisms (Caenorhabditis elegans [5] & Pristionchus pacificus [6]), elucidation of subcellular fermentation pathways in Saccharomyces cerevisiae [7], determined the intracellular processing of foreign material in human mesenchymal stem cells (hMSCs) [8] & characterised the evolution of acid by-products during bacterial biofilm growth [9].This project will continue the application of pH-sensitive fluorescent nanosensors to diverse biological environments, which include fruit flies (Drosophila melanogaster), Thale cress (Arabidopsis thaliana) and CHO/Heck 293 cell culture for bioprocessing. As well as develop new biosensors for key biological molecules and ions.

荧光纳米传感器是一种惰性的多功能生物传感器，可用于在生命界面的物理学中对微环境中的关键分子和离子进行重要测量，并且是动态过程实时测量的理想选择[1]。在诺丁汉大学，荧光纳米传感器已被开发用于量化复杂模型系统中的pH值[2]、分子氧[3]和温度[4]。在这些传感器中，pH敏感的荧光纳米传感器聚集了最大的动力。它们已经在一系列复杂多样的微环境中进行了评估和验证，并通过绘制线虫模式生物的酸化图展示了它们的巨大潜力（秀丽隐杆线虫[5] &太平洋杜父线虫[6]），阐明酿酒酵母中的亚细胞发酵途径[7]，确定了人类间充质干细胞（hMSCs）中异物的细胞内处理[8]，并表征了细菌生物膜生长过程中酸性副产物的演变[9]该项目将继续将pH敏感荧光纳米传感器应用于各种生物环境，包括果蝇（Drosophila melanogaster）、塔勒（Arabidopsis thaliana）和CHO/Heck 293细胞培养物用于生物加工。以及为关键的生物分子和离子开发新的生物传感器。