Towards an understanding of olfactory adaptation

了解嗅觉适应

• 批准号: 7112920
• 负责人: Jin Il Lee
• 金额: $ 3.07万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-01 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7112920
• 关键词: Caenorhabditis elegans; alternatives to animals in research; biological signal transduction; calcium channel; chemotaxis; helminth genetics; immunocytochemistry; intermolecular interaction; learning; neural plasticity; neurogenetics; neurons; neuroregulation; odors; olfactions; olfactory threshold; potassium channel; predoctoral investigator; protein localization; protein structure function; serine threonine protein kinase

DESCRIPTION (provided by applicant): The goal of this project is to understand how repeated experience can stably modify a neuron's activity by studying the molecules that affect the nuclear localization of the cGMP-dependent protein kinase EGL-4 during long-term odorant adaptation in C. elegans. This understanding will provide us with insight into mechanisms of learning and memory and molecular processes that underlie addictions. Nuclear localization of EGL-4 in odorant sensory neurons is necessary for the transition from short to long-term adaptation that occurs upon continual stimulation to an odorant. We propose to study the molecules that regulate this nuclear translocation. By utilizing a GFP-tagged EGL-4 molecule to visualize in real-time the behavior of EGL-4, we will examine the localization of EGL-4 in known components of both odorant chemotaxis and adaptation. We will also genetically screen for individuals that are defective in EGL-4 nuclear translocation to identify new components of adaptation that regulate the transition to long-term adaptation. The results from this study will give us a clearer understanding of neuronal plasticity.

描述（由申请人提供）：该项目的目标是通过研究在线虫长期气味适应过程中影响 cGMP 依赖性蛋白激酶 EGL-4 核定位的分子，了解重复的经验如何稳定地改变神经元的活动。这种理解将使我们深入了解成瘾背后的学习和记忆机制以及分子过程。 EGL-4 在气味感觉神经元中的核定位对于在持续刺激气味时发生的从短期适应到长期适应的转变是必要的。我们建议研究调节这种核易位的分子。通过利用 GFP 标记的 EGL-4 分子实时可视化 EGL-4 的行为，我们将检查 EGL-4 在气味趋化和适应的已知成分中的定位。我们还将对 EGL-4 核易位缺陷的个体进行基因筛查，以确定调节向长期适应过渡的新适应成分。这项研究的结果将使我们对神经元可塑性有更清晰的了解。