DNA Microarray Analysis of Neuronal Excitability
神经元兴奋性的 DNA 微阵列分析
基本信息
- 批准号:7066048
- 负责人:
- 金额:$ 16.08万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2005
- 资助国家:美国
- 起止时间:2005-05-15 至 2010-04-30
- 项目状态:已结题
- 来源:
- 关键词:amphetaminesbehavioral /social science research tagcocainedrug addictiondrug receptorsgene expressiongene expression profilinghigh throughput technologyhippocampuslaboratory mouselaboratory ratmembrane channelsmicroarray technologyneural conductionneural information processingneural plasticityneurogeneticsnucleus accumbensprefrontal lobe /cortexpyramidal cellssingle cell analysisvoltage /patch clampvoltage gated channel
项目摘要
DESCRIPTION (provided by applicant): The goal of this proposal is to establish a genetic profile of neuronal excitability as it relates to psychostimulant addiction. To accomplish this aim we will be using the most advanced oligonucleotide high density microarray technology combined with patch-clamp electrophysiology. Affymetrix Gene Chips allow for genome wide scans of the entire mouse genome for genes susceptible to chronic psychostimulant administration. In order to understand neural information procession in the brain reward circuitry as it relates to addiction, it is necessary to understand how individual neurons within this system code reward-related information. This project uses both DNA microarrays and patch-clamp electrophysiology to establish a molecular and functional characterization of nucleus accumbens, prefrontal cortical pyramidal neurons and ventral subicular hippocampal pyramidal neurons after repeated psychomotor stimulant injections in rats and mice. Specific aim 1 is to look for voltage and ligand-gated ion-channel genes that are subject to modulation by psychostimulants in the accumbens, subiculum and prefrontal cortical neurons. Specific aim 2 is to determine the functional manifestations of those changes within each structure. Specific aim 3 involves individual neuron gene expression profiling of electrophysiologically classified neurons (e.g. Interneurons and pyramidal bursting and nonbursting). Together these experiments will help us understand how drugs of abuse induce long-lasting neuroadaptations within the brain reward circuitry. With greater understanding of addiction-associated plasticity it may be possible to develop pharmacological or gene profiling strategies to screen for addiction vulnerability and possible therapeutic interventions.
描述(由申请人提供):该提案的目标是建立与精神兴奋剂成瘾相关的神经元兴奋性的遗传特征。为了实现这一目标,我们将使用最先进的寡核苷酸高密度微阵列技术与膜片钳电生理学相结合。 Affymetrix 基因芯片允许对整个小鼠基因组进行全基因组扫描,寻找对长期精神兴奋剂给药敏感的基因。为了了解大脑奖励回路中与成瘾相关的神经信息处理,有必要了解该系统中的各个神经元如何编码奖励相关信息。该项目利用 DNA 微阵列和膜片钳电生理学,对大鼠和小鼠重复注射精神运动兴奋剂后,建立伏隔核、前额皮质锥体神经元和腹侧下皮层锥体神经元的分子和功能表征。具体目标1是寻找电压和配体门控离子通道基因,这些基因在伏隔核、下托和前额皮质神经元中受到精神兴奋剂的调节。具体目标 2 是确定每个结构内这些变化的功能表现。具体目标 3 涉及电生理学分类神经元(例如中间神经元和锥体爆裂和非爆裂)的个体神经元基因表达谱。这些实验将帮助我们了解滥用药物如何在大脑奖励回路中诱导持久的神经适应。随着对成瘾相关可塑性的深入了解,有可能开发出药理学或基因分析策略来筛选成瘾脆弱性和可能的治疗干预措施。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
数据更新时间:{{ journalArticles.updateTime }}
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
DONALD C COOPER其他文献
DONALD C COOPER的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('DONALD C COOPER', 18)}}的其他基金
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
7480823 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Pathway specific ecstasy-induced plasticity of excitability in the subiculum
途径特异性摇头丸诱导的下托兴奋性可塑性
- 批准号:
7636741 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
7586641 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
8079336 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
7765483 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
8033756 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
7907290 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Pathway specific ecstasy-induced plasticity of excitability in the subiculum
途径特异性摇头丸诱导的下托兴奋性可塑性
- 批准号:
7536111 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
Plasticity of excitability in ventral subiculum after high cocaine intake
高可卡因摄入后腹侧下托兴奋性的可塑性
- 批准号:
8220831 - 财政年份:2008
- 资助金额:
$ 16.08万 - 项目类别:
DNA Microarray Analysis of Neuronal Excitability
神经元兴奋性的 DNA 微阵列分析
- 批准号:
7990916 - 财政年份:2005
- 资助金额:
$ 16.08万 - 项目类别: