Application of Genomic Microarrays to Breast Cancer

基因组微阵列在乳腺癌中的应用

• 批准号: 7066088
• 负责人: ROBERT LUCITO
• 金额: $ 14.62万
• 依托单位: COLD SPRING HARBOR LABORATORY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-06-01 至 2007-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7066088
• 关键词: breast neoplasm /cancer diagnosis; breast neoplasms; chromosome aberrations; clinical research; flow cytometry; gene expression; genetic polymorphism; genome; human tissue; laser capture microdissection; linkage mapping; loss of heterozygosity; microarray technology; neoplasm /cancer classification /staging; neoplasm /cancer genetics; neoplastic process; neoplastic transformation; nucleic acid hybridization; prognosis

DESCRIPTION (provided by applicant): It is my goal to utilize this funding mechanism to answer a biological question. In the process I will be expanding my knowledge base, by taking courses and workshops in the field of bioinformatics, a discipline that my project relies on heavily, and the field of pathobiology, information I feel will give me a better understanding of the cancers that I intend on studying. I intend to use an array-based method to determine genome copy number fluctuations between two cancer genomes. This method based on genomic representations has been shown to be accurate at detecting deletions and amplifications from primary breast tumors. This method will be used to study the genome copy number fluctuations that occur in primary breast cancer. Furthermore it will be used to compare the copy number fluctuations between ductal carcinoma in situ (DCIS), and invasive ductal carcinoma (IDC). The regions that undergo copy number fluctuations in DCIS will be compared to those in IDC for similarity, to demonstrate whether IDC derives from DCIS. Copy number fluctuations identified for all tumor biopsies will be mapped to a finer resolution than before possible. The regions of the genome identified may represent regions, when mutated contribute to the path to malignancy. These regions will have several uses as markers, for diagnosis, prognosis, and for the identification of gene candidates involved in the path to malignancy.

描述（由申请人提供）：我的目标是利用这笔资金

项目成果

Methylation detection oligonucleotide microarray analysis: a high-resolution method for detection of CpG island methylation.

• DOI: 10.1093/nar/gkp413
• 发表时间: 2009-07
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Kamalakaran S;Kendall J;Zhao X;Tang C;Khan S;Ravi K;Auletta T;Riggs M;Wang Y;Helland A;Naume B;Dimitrova N;Børresen-Dale AL;Hicks J;Lucito R
• 通讯作者: Lucito R